Our Center for the Structural Biology of Cellular Host Elements in Egress, Trafficking, and Assembly of HIV (CHEETAH) studies the structural biology of HIV-Host interactions involved in viral trafficking and assembly (as broadly defined). Our biological studies will focus on the structural biology, biochemistry, cell biology and molecular virology of five key aspects of the HIV-1 life cycle: 1) Reverse transcription and preintegration complex transformations and trafficking, 2) TRIM5a restriction, 3) Rev-dependent DEAD-box helicase transformations, 4) Virion assembly and budding, and 5) Virion structure, energetics and maturation. In each case, we aim to understand: 1) The molecular machines that drive these events, 2) The mechanisms by which host pathways are recruited and utilized, and 3) The underlying viral and cellular structures. In parallel, we will pursue technological developments in four different areas that will facilitate our biological studies and are important frontiers in HIV research: 1) Single-molecule analyses of native Rev complexes, 2) Multiscale computer simulations of viral capsid structure and assembly, 3) Advances in virus imaging, and 4) Development and application of methodology for imaging HIV in tissues. The efforts of our Center will also be extended through: 1) Extensive collaborations with other PSO Centers and colleagues, and access to scientific resources within the biomedical community, 2) A Collaborative Development Program that will fund promising collaborators who will enhance and benefit from interactions with our Center, and 3) Training programs for younger scientists. Our overall goals are to lay the groundwork for development of new antiviral strategies and to continue to develop HIV into an unparalleled model system for studying how a human virus interacts with its host.
Like other viruses, HIV-1 makes extensive use of host factors and pathways as it traffics through the cell and undergoes the transformations associated with each stage of the viral life cycle. These virus-host interfaces are, in principle, attractive targes for therapeutic intervention. Our program will inform efforts to develop this promise by providing a more comprehensive understanding of the structures, mechanisms, and functions of critical host-virus interactions.
|Bailey, Lucas J; Sheehy, Kimberly M; Dominik, Pawel K et al. (2017) Locking the Elbow: Improved Antibody Fab Fragments as Chaperones for Structure Determination. J Mol Biol :|
|Hammond, John A; Zhou, Li; Lamichhane, Rajan et al. (2017) A Survey of DDX21 Activity During Rev/RRE Complex Formation. J Mol Biol :|
|Sdano, Matthew A; Fulcher, James M; Palani, Sowmiya et al. (2017) A novel SH2 recognition mechanism recruits Spt6 to the doubly phosphorylated RNA polymerase II linker at sites of transcription. Elife 6:|
|Kieffer, Collin; Ladinsky, Mark S; Ninh, Allen et al. (2017) Longitudinal imaging of HIV-1 spread in humanized mice with parallel 3D immunofluorescence and electron tomography. Elife 6:|
|Mamede, João I; Cianci, Gianguido C; Anderson, Meegan R et al. (2017) Early cytoplasmic uncoating is associated with infectivity of HIV-1. Proc Natl Acad Sci U S A 114:E7169-E7178|
|Lamichhane, Rajan; Hammond, John A; Pauszek 3rd, Raymond F et al. (2017) A DEAD-box protein acts through RNA to promote HIV-1 Rev-RRE assembly. Nucleic Acids Res 45:4632-4641|
|Monroe, Nicole; Han, Han; Shen, Peter S et al. (2017) Structural basis of protein translocation by the Vps4-Vta1 AAA ATPase. Elife 6:|
|Gu, Mingyu; LaJoie, Dollie; Chen, Opal S et al. (2017) LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells. Proc Natl Acad Sci U S A 114:E2166-E2175|
|Freund, Natalia T; Wang, Haoqing; Scharf, Louise et al. (2017) Coexistence of potent HIV-1 broadly neutralizing antibodies and antibody-sensitive viruses in a viremic controller. Sci Transl Med 9:|
|Pak, Alexander J; Grime, John M A; Sengupta, Prabuddha et al. (2017) Immature HIV-1 lattice assembly dynamics are regulated by scaffolding from nucleic acid and the plasma membrane. Proc Natl Acad Sci U S A 114:E10056-E10065|
Showing the most recent 10 out of 160 publications