Our overall goal is to systematically define c/s-regulatory elements in mRNAs that control miRNA-mediated silencing under defined physiological conditions. While many miRNA targets have been predicted, and some individual targets have been investigated experimentally, the ways in which mRNA structures enhance or inhibit miRNA targeting are not known for the human transcriptome.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center (P50)
Project #
1P50GM102706-01
Application #
8516179
Study Section
Special Emphasis Panel (ZGM1-CBCB-3 (SB))
Project Start
Project End
Budget Start
2012-09-01
Budget End
2013-08-31
Support Year
1
Fiscal Year
2012
Total Cost
$421,196
Indirect Cost
$150,620
Name
University of California Berkeley
Department
Type
DUNS #
124726725
City
Berkeley
State
CA
Country
United States
Zip Code
94704
Smith, M Duane; Arake-Tacca, Luisa; Nitido, Adam et al. (2016) Assembly of eIF3 Mediated by Mutually Dependent Subunit Insertion. Structure 24:886-96
Nguyen, Duy P; Miyaoka, Yuichiro; Gilbert, Luke A et al. (2016) Ligand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity. Nat Commun 7:12009
Dunn, Joshua G; Weissman, Jonathan S (2016) Plastid: nucleotide-resolution analysis of next-generation sequencing and genomics data. BMC Genomics 17:958
Oh, Sekyung; Flynn, Ryan A; Floor, Stephen N et al. (2016) Medulloblastoma-associated DDX3 variant selectively alters the translational response to stress. Oncotarget 7:28169-82
Choudhary, Krishna; Shih, Nathan P; Deng, Fei et al. (2016) Metrics for rapid quality control in RNA structure probing experiments. Bioinformatics 32:3575-3583
Adamson, Britt; Norman, Thomas M; Jost, Marco et al. (2016) A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response. Cell 167:1867-1882.e21
Lee, Amy S; Kranzusch, Philip J; Doudna, Jennifer A et al. (2016) eIF3d is an mRNA cap-binding protein that is required for specialized translation initiation. Nature 536:96-9
Horlbeck, Max A; Gilbert, Luke A; Villalta, Jacqueline E et al. (2016) Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation. Elife 5:
Iwasaki, Shintaro; Floor, Stephen N; Ingolia, Nicholas T (2016) Rocaglates convert DEAD-box protein eIF4A into a sequence-selective translational repressor. Nature 534:558-61
Gonzalez, Tania L; Liang, Yan; Nguyen, Bao N et al. (2015) Tight regulation of plant immune responses by combining promoter and suicide exon elements. Nucleic Acids Res 43:7152-61

Showing the most recent 10 out of 33 publications