The Neuroanatomy Core will provide both light and electron microscopic services to the Projects, including tissue staining, sectioning and imaging.
Ten aims i n three projects will utilize this Core facility. Projects 2( Rosenberg) and 3 (Saper) will use the Core for in situ hybridization histochemistry to localize mRNA for phosphodiesterases, adenosine receptors and glutamic acid decarboxylase in rat brain, and the latter also in human brain. Light microscopic immunocytochemical services will be used by Project 2, 3, and 4 to localize cholinergic neurons, as well as immunocytochemistry for neurons containing enkephalin, in some cases combined with retrograde tract tracing or in situ hybridization methods. Electron microscopic immunocytochemistry will be used by Project 4 to examine the chemical specificity of afferents to hypoglossal motor neurons. The centralization of these services in one laboratory will give the laboratories that do relative little neuroanatomy (Rosenberg and Greene) access to the technical proficiency of the Core laboratory and staff and will allow economy of scale in order and using reagents, isotopes and photographic emulsion.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Specialized Center (P50)
Project #
1P50HL060292-01
Application #
6110970
Study Section
Project Start
1998-09-01
Project End
1999-08-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
1
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Harvard University
Department
Type
DUNS #
082359691
City
Boston
State
MA
Country
United States
Zip Code
02115
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