Project 3 incorporates two distinct studies, each of which capitalizes on extensive longitudinal, affective behavioral phenotyping during infancy or early childhood to study early adult outcomes based on neuroimaging (Study 1) or induced pluripotent stem cells. Study 1's monozygotic (MZ) twin difference design allows us to distinguish whether observed differences in later brain structure and function are associated with environmental influences versus familial influences on early anxious temperament. The comparison of MZ cotwins, with their age-matched, grossly similar brain morphology, allows detection of more subtle MRI effects in humans than any competing non-experimental design. Study 2 is a pilot study that is coordinated with the nonhuman primate stem cell work in Project 1. We examine whether young adults who are slow in amygdala recovery to negative affect elicitation and chronically anxious differ from those selected to be rapid in amygdala recovery and non-anxious differ in gene expression and cellular electrophysiology of GABAergic neurons that are derived from induced pluripotent stem cells.

Public Health Relevance

Understanding the early origins as well as the brain bases for conditions such as anxiety and depression is an important priority for public health. These issues can be addressed in studies that carefully assess behavior and brain structure and function beginning in infancy and extending through adolescence. Employing identical twins in this research allows control of genetic differences that affect development.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Specialized Center (P50)
Project #
5P50MH100031-02
Application #
8727674
Study Section
Special Emphasis Panel (ZMH1)
Project Start
Project End
Budget Start
2014-09-01
Budget End
2015-08-31
Support Year
2
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
DUNS #
City
Madison
State
WI
Country
United States
Zip Code
53715
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