This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The primary goal of the model remains the same as in the original protocol. This project is focused on creating genomic/proteomic resources that can be applied to research using nonhuman primates. Through the construction of proteomic resources, we intend to provide the research community with the full complement of species-specific genomic and proteomic tools needed to apply the technologies of functional genomics to nonhuman primate research. We will use sensitive, high-resolution mass spectrometry to build databases of identified proteins present in various rhesus macaque (Macaca mulatta) tissues. These databases will be used to characterize the changes in cellular protein profiles that occur in response to simian immunodeficiency virus infection. These studies will allow us to evaluate these resources in an experimental system that will enhance the use of macaques as models for AIDS research. The results will be shared via Web portals, as a resource to enable researchers to maximize the types and amounts of information that can be obtained from these valuable research animals. We will perform a systems biology interrogation of an acute mucosal infection model, employing intra-rectal challenge with SIVmac251 in 24 male, Indian-origin rhesus macaques. The primary hypothesis is that a detailed systems level analysis of early changes in the acute infection with a pathogenic strain of SIV will reveal events in innate immunity, inflammatory processes, and the ensuing transition to the adaptive immune response. As one corollary to this hypothesis, these features may serve as prognosticators of outcome, as reflected in the variability of individual animals. A second corollary is that such a systems level analysis will identify specific targets and pathways for intervention by pharmacologic agents or vaccine modalities

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Primate Research Center Grants (P51)
Project #
5P51RR000166-50
Application #
8357630
Study Section
Special Emphasis Panel (ZRR1-CM-8 (02))
Project Start
2011-05-01
Project End
2012-04-30
Budget Start
2011-05-01
Budget End
2012-04-30
Support Year
50
Fiscal Year
2011
Total Cost
$377,899
Indirect Cost
Name
University of Washington
Department
Veterinary Sciences
Type
Other Domestic Higher Education
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
McAdams, Ryan M; McPherson, Ronald J; Kapur, Raj P et al. (2017) Focal Brain Injury Associated with a Model of Severe Hypoxic-Ischemic Encephalopathy in Nonhuman Primates. Dev Neurosci 39:107-123
Klegarth, Amy R; Ezeonwu, Chigozie A; Rompis, Aida et al. (2017) Survey of Treponemal Infections in Free-Ranging and Captive Macaques, 1999-2012. Emerg Infect Dis 23:816-819
Hallum, Luke E; Shooner, Christopher; Kumbhani, Romesh D et al. (2017) Altered Balance of Receptive Field Excitation and Suppression in Visual Cortex of Amblyopic Macaque Monkeys. J Neurosci 37:8216-8226
Seeman, Stephanie C; Mogen, Brian J; Fetz, Eberhard E et al. (2017) Paired Stimulation for Spike-Timing-Dependent Plasticity in Primate Sensorimotor Cortex. J Neurosci 37:1935-1949
Balakrishnan, Ashwini; Goodpaster, Tracy; Randolph-Habecker, Julie et al. (2017) Analysis of ROR1 Protein Expression in Human Cancer and Normal Tissues. Clin Cancer Res 23:3061-3071
Shooner, Christopher; Hallum, Luke E; Kumbhani, Romesh D et al. (2017) Asymmetric Dichoptic Masking in Visual Cortex of Amblyopic Macaque Monkeys. J Neurosci 37:8734-8741
Rembado, Irene; Zanos, Stavros; Fetz, Eberhard E (2017) Cycle-Triggered Cortical Stimulation during Slow Wave Sleep Facilitates Learning a BMI Task: A Case Report in a Non-Human Primate. Front Behav Neurosci 11:59
Richter, Maximilian; Yumul, Roma; Saydaminova, Kamola et al. (2016) Preclinical safety, pharmacokinetics, pharmacodynamics, and biodistribution studies with Ad35K++ protein: a novel rituximab cotherapeutic. Mol Ther Methods Clin Dev 5:16013
McLoon, Linda K; Christiansen, Stephen P; Ghose, Geoffrey M et al. (2016) Improvement of Eye Alignment in Adult Strabismic Monkeys by Sustained IGF-1 Treatment. Invest Ophthalmol Vis Sci 57:6070-6078
Liao, Hsi-Wen; Ren, Xiaozhi; Peterson, Beth B et al. (2016) Melanopsin-expressing ganglion cells on macaque and human retinas form two morphologically distinct populations. J Comp Neurol 524:2845-72

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