The mission of the Hormone Assay Core are to provide cost effective and efficient analyses of a variety of hormones, appropriate methodologies, cell lines and data storage venues to promote the Diabetes research efforts of the Einstein DRTC. Over the past two years, the Core has averaged 30,000 assays annually in support of 34 DRTC investigators. Productivity of the Core has been outstanding with 76 papers published that involved Core usage in the past 2.5 years. In addition, the appropriate physiological secretory function and insulin content of a variety of rodent mouse beta cell lines are characterized prior to supplying them to investigators at Einstein and to other investigator laboratories nationally and internationally. Thus, this Core has functioned as a local and national resource in support of diabetes-related research. The Core utilizies RIA, ELISA, and Luminex-based methodologies that have been established by the Core director and personnel. Data are pemianently archived and the results of assays are made available via a secure website accessible by investigators. These data are encrypted and access to the server is password protected. A charge back system is used to recover costs not covered by the DRTC budget. Education of investigators on the appropriate assay method is the function of the Director, and laboratory personnel instruct fellows and post-docs in the methodologies when appropriate. To accomplish these goals, the Hormone Assay Core will: 1) provide accurate, cost effective assays of a variety of hormones, adipokines and cytokines to DRTC investigators, using appropriate methodologies;2) assess insulin secretion from a series of mouse beta cell lines maintained by the Einstein DRTC for local use and distribution to the scientific community;3) provide an effective data storage and access system for providing results to investigators and monitoring Core use; 4) provide expert advice to DRTC investigators on Core use and education in assay performance and data interpretation;and 5) utilize the Core as a training platform for appropriate young diabetes investigators. Future goals are to enhance assay automation through shared purchase of a robot and bar-code compatible gamma counter, which will be used to enhance semi-automation of procedures. All these sen/ices are available to investigators new to diabetes research, as well as to investigators working on diabetes-related projects that can be enriched and extended by the use of the expertise and facilities of this core.
The Hormone Assay Core provides cost effective, high quality assays that facilitate the scientific progress made by a large group of scientists committed to improve our understanding and treatment of patients with diabetes.
|Rudolph, Bryan; Bjorklund, Nicole; Ovchinsky, Nadia et al. (2018) Methods to improve the noninvasive diagnosis and assessment of disease severity in children with suspected nonalcoholic fatty liver disease (NAFLD): Study design. Contemp Clin Trials 75:51-58|
|Tang, Yan; Kwon, Hyokjoon; Neel, Brian A et al. (2018) The fructose-2,6-bisphosphatase TIGAR suppresses NF-?B signaling by directly inhibiting the linear ubiquitin assembly complex LUBAC. J Biol Chem 293:7578-7591|
|Chemaly, Elie R; Troncone, Luca; Lebeche, Djamel (2018) SERCA control of cell death and survival. Cell Calcium 69:46-61|
|Iqbal, Niloy Jafar; Lu, Zhonglei; Liu, Shun Mei et al. (2018) Cyclin-dependent kinase 4 is a preclinical target for diet-induced obesity. JCI Insight 3:|
|Schloss, Jennifer; Ali, Riyasat; Racine, Jeremy J et al. (2018) HLA-B*39:06 Efficiently Mediates Type 1 Diabetes in a Mouse Model Incorporating Reduced Thymic Insulin Expression. J Immunol 200:3353-3363|
|Racine, Jeremy J; Stewart, Isabel; Ratiu, Jeremy et al. (2018) Improved Murine MHC-Deficient HLA Transgenic NOD Mouse Models for Type 1 Diabetes Therapy Development. Diabetes 67:923-935|
|Shu, Jun; Santulli, Gaetano (2018) Update on peripheral artery disease: Epidemiology and evidence-based facts. Atherosclerosis 275:379-381|
|Zhao, Xiaoping; Zhao, Li; Yang, Hao et al. (2018) Pyruvate kinase M2 interacts with nuclear sterol regulatory element-binding protein 1a and thereby activates lipogenesis and cell proliferation in hepatocellular carcinoma. J Biol Chem 293:6623-6634|
|Qiu, Yunping; Moir, Robyn D; Willis, Ian M et al. (2018) Enhanced Isotopic Ratio Outlier Analysis (IROA) Peak Detection and Identification with Ultra-High Resolution GC-Orbitrap/MS: Potential Application for Investigation of Model Organism Metabolomes. Metabolites 8:|
|Liu, Shunmei; Marcelin, Genevieve; Blouet, Clemence et al. (2018) A gut-brain axis regulating glucose metabolism mediated by bile acids and competitive fibroblast growth factor actions at the hypothalamus. Mol Metab 8:37-50|
Showing the most recent 10 out of 559 publications