The mission of the Flow Cytometry Core is to assist investigators in performing multi-parameter analysis and high speed sorting of cell populations based on labeling with fluorochrome conjugated antibodies or other fluorescent dye reagents. In addition to providing advanced flow cytometry equipment and technical expertise for investigators, the Core also provides training to guide DRTC investigators and their staff in the application of basic and advanced flow cytometry techniques. The analytical and preparative cell analyses provided by this Core allows DRTC investigators to identify specific changes in cell population that are associated with metabolic dysfunction in human and rodent models. The expertise and knowledgeable staff of the Flow Cytometry Core provides investigators a wide range of specialized, high quality methodologies and tools relevant to understanding the adaptive and innate immune systems related to metabolic regulation as well as developmental and regenerative studies that are now recognized as important components of diabetes and obesity. To accomplish these goals, the Flow Cytometry Core will: 1) advise investigators, trainees and staff on the utility of FACS for their specific research problem;2) make available to investigators specialized flow cytometry analyses for the quantification of cells expressing specific surface markers or undergoing specific cellular signaling events;3) make available high-speed flow-based flourescence activated cell sorting for the sterile isolation of cells displaying specific cell surface markers or biological properties;4) provide laboratory training of students, postdoctoral fellows, investigators and technical staff in performing techniques necessary to perfonn fluorescent-based cell analyses and cell sorting;and 5) assist in the appropriate data analysis for the evaluation of molecular targets and biological processes. All these services are available to investigators new to diabetes research, as well as to investigators working on diabetes-related projects that can be enriched and extended by the use of the expertise and facilities of this core.

Public Health Relevance

The mission of the Flow Cytometry Core is to assist investigators in performing multi-parameter analysis and high speed sorting of cell populations based on labeling with fluorochrome conjugated antibodies or other fluorescent dye reagents. In addition to providing advanced flow cytometry equipment and technical expertise for investigators, the Core also provides training to guide DRTC investigators and their staff in the application of basic and advanced flow cytometry techniques. The analytical and preparative cell analyses provided by this Core allows DRTC investigators to identify specific changes in cell population that are associated with metabolic dysfunction in human and rodent models. The expertise and knowledgeable staff of the Flow Cytometry Core provides investigators a wide range of specialized, high quality methodologies and tools relevant to understanding the adaptive and innate immune systems related to metabolic regulation as well as developmental and regenerative studies that are now recognized as important components of diabetes and obesity. To accomplish these goals, the Flow Cytometry Core will: 1) advise investigators, trainees and staff on the utility of FACS for their specific research problem;2) make available to investigators specialized flow cytometry analyses for the quantification of cells expressing specific surface markers or undergoing specific cellular signaling events;3) make available high-speed flow-based flourescence activated cell sorting for the sterile isolation of cells displaying specific cell surface markers or biological properties;4) provide laboratory training of students, postdoctoral fellows, investigators and technical staff in performing techniques necessary to perfonn fluorescent-based cell analyses and cell sorting;and 5) assist in the appropriate data analysis for the evaluation of molecular targets and biological processes. All these services are available to investigators new to diabetes research, as well as to investigators working on diabetes-related projects that can be enriched and extended by the use of the expertise and facilities of this

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Comprehensive Center (P60)
Project #
5P60DK020541-37
Application #
8637044
Study Section
Special Emphasis Panel (ZDK1-GRB-2)
Project Start
Project End
Budget Start
2014-04-01
Budget End
2015-03-31
Support Year
37
Fiscal Year
2014
Total Cost
$21,763
Indirect Cost
$8,653
Name
Albert Einstein College of Medicine
Department
Type
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
Lawman, Hannah G; Mallya, Giridhar; Veur, Stephanie Vander et al. (2015) Trends in relative weight over 1 year in low-income urban youth. Obesity (Silver Spring) 23:436-42
Conlon, Beth A; Kahan, Michelle; Martinez, Melissa et al. (2015) Development and Evaluation of the Curriculum for BOLD (Bronx Oncology Living Daily) Healthy Living: a Diabetes Prevention and Control Program for Underserved Cancer Survivors. J Cancer Educ 30:535-45
Lent, Michelle R; Vander Veur, Stephanie; Mallya, Giridhar et al. (2015) Corner store purchases made by adults, adolescents and children: items, nutritional characteristics and amount spent. Public Health Nutr 18:1706-12
Goyal, Akankasha; Nimmakayala, Kameswara Rao; Zonszein, Joel (2014) Is there a paradox in obesity? Cardiol Rev 22:163-70
Beasley, Jeannette M; Gunter, Marc J; LaCroix, Andrea Z et al. (2014) Associations of serum insulin-like growth factor-I and insulin-like growth factor-binding protein 3 levels with biomarker-calibrated protein, dairy product and milk intake in the Women's Health Initiative. Br J Nutr 111:847-53
Cuervo, Ana Maria; Wong, Esther (2014) Chaperone-mediated autophagy: roles in disease and aging. Cell Res 24:92-104
Abdulla, Arian; Zhang, Yi; Hsu, Fu-Ning et al. (2014) Regulation of lipogenic gene expression by lysine-specific histone demethylase-1 (LSD1). J Biol Chem 289:29937-47
Holmes, Michael V; Lange, Leslie A; Palmer, Tom et al. (2014) Causal effects of body mass index on cardiometabolic traits and events: a Mendelian randomization analysis. Am J Hum Genet 94:198-208
Bashiri, Asher; Heo, Hye J; Ben-Avraham, Danny et al. (2014) Pregnancy complicated by obesity induces global transcript expression alterations in visceral and subcutaneous fat. Mol Genet Genomics 289:695-705
Schneider, Jaime L; Suh, Yousin; Cuervo, Ana Maria (2014) Deficient chaperone-mediated autophagy in liver leads to metabolic dysregulation. Cell Metab 20:417-32

Showing the most recent 10 out of 404 publications