The adverse consequences of obesity are pleiotrophic for the mammalian organism. Animal models of obesity that allow careful characterization of the pervasive metabolic effects of the disease are vital to the success of our mission. To enhance research into the causes and consequences of obesity, we established a mouse phenotyping core. The primary goals of this core are to develop and provide standardized state-ofthe- art methods for the analysis of animal models of disease. The core applies the analytical expertise of ten laboratories to provide histology, neuroanatomy, gene expression, mass spectrometry, physiology, lipid, glucose, metabokine, and behavioral assays. The core is directed by Russell, Elmquist, and Scherer and is staffed by a dedicated team of skilled technicians who utilize existing equipment to perform optimized assays on samples provided by TORS investigators. Currently available assays include histological staining and antigen detection, in situ mRNA hybridization, real time reverse transcriptase-PCR, microarray analysis, laser capture microdissection, small molecule mass spectrometry and metabolite profiling, metabolic cage analyses, complete lipid balance studies, magnetic resonance and computed tomography imaging, glucose tolerance and clamp analyses, serum hormone measurements, routine blood chemistries, exhaustive behavioral analyses, and synaptic plasticity measurements in brain slices. As new research findings dictate, novel methodologies are developed and made available by the core. For each experiment, results are collected, calculated, and compiled in electronic format and returned to submitting investigators. The availability of the services provided by this core accelerates the pace of our research, allows centralized troubleshooting, and facilitates the efficient utilization of existing expertise and resources. The mouse phenotyping core is a crucial component of our Roadmap effort that brings together a diverse team of scientists and clinicians to ensure that discoveries made in obesity research are rapidly translated into advances in patient care.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Linked Center Core Grant (PL1)
Project #
5PL1DK081182-05
Application #
8139678
Study Section
Special Emphasis Panel (ZRR1-SRC (99))
Program Officer
Laughlin, Maren R
Project Start
2007-09-30
Project End
2013-06-30
Budget Start
2011-07-01
Budget End
2013-06-30
Support Year
5
Fiscal Year
2011
Total Cost
$370,840
Indirect Cost
Name
University of Texas Sw Medical Center Dallas
Department
Genetics
Type
Schools of Medicine
DUNS #
800771545
City
Dallas
State
TX
Country
United States
Zip Code
75390
Udit, Swalpa; Burton, Michael; Rutkowski, Joseph M et al. (2017) Nav1.8 neurons are involved in limiting acute phase responses to dietary fat. Mol Metab 6:1081-1091
Gupta, Arjun; Pandey, Ambarish; Ayers, Colby et al. (2017) An Analysis of Individual Body Fat Depots and Risk of Developing Cancer: Insights From the Dallas Heart Study. Mayo Clin Proc 92:536-543
Yao, Ting; Deng, Zhuo; Gao, Yong et al. (2017) Ire1? in Pomc Neurons Is Required for Thermogenesis and Glycemia. Diabetes 66:663-673
Witberg, Guy; Ayers, Colby R; Turer, Aslan T et al. (2016) Relation of Adiponectin to All-Cause Mortality, Cardiovascular Mortality, and Major Adverse Cardiovascular Events (from the Dallas Heart Study). Am J Cardiol 117:574-579
Neeland, Ian J; Turer, Aslan T; Ayers, Colby R et al. (2015) Body fat distribution and incident cardiovascular disease in obese adults. J Am Coll Cardiol 65:2150-1
Xing, Frank Y F; Neeland, Ian J; Gore, M Odette et al. (2014) Association of prediabetes by fasting glucose and/or haemoglobin A1c levels with subclinical atherosclerosis and impaired renal function: observations from the Dallas Heart Study. Diab Vasc Dis Res 11:11-8
Grundy, Scott M; Neeland, Ian J; Turer, Aslan T et al. (2014) Ethnic and gender susceptibility to metabolic risk. Metab Syndr Relat Disord 12:110-6
Liu, Chen; Bookout, Angie L; Lee, Syann et al. (2014) PPAR? in vagal neurons regulates high-fat diet induced thermogenesis. Cell Metab 19:722-30
Williams, Kevin W; Liu, Tiemin; Kong, Xingxing et al. (2014) Xbp1s in Pomc neurons connects ER stress with energy balance and glucose homeostasis. Cell Metab 20:471-82
Kohno, Daisuke; Lee, Syann; Harper, Matthew J et al. (2014) Dnmt3a in Sim1 neurons is necessary for normal energy homeostasis. J Neurosci 34:15288-96

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