The overall goal of this research and training plan is to gain better understanding of the organization and function of the mouse accessory olfactory system (AOS). The AOS is a compact neural pathway that communicates directly with brain regions that govern so-called "intrinsic" social and reproductive behaviors. By increasing knowledge about sensory processing in the mouse AOS, this project will shed light on principles of mammalian olfaction, and will improve our understanding of links between sensation and behavior. The investigator, Dr. Julian Meeks, will gain up to two years of advanced postdoctoral training in the design and implementation of planar illumination microscopy and transgenic mice in support of an innovative approach using live neuronal calcium imaging to study inhibitory interneurons in the mouse accessory olfactory bulb. Two sponsors for this training were chosen, Dr. Timothy Holy and Dr. Daniel Kerschensteiner, that have strong experience in the fields of optical design and transgenic mouse production, respectively. Several hypotheses will be tested during the mentored and independent phases of this award. One main hypothesis links sensory "receptive fields" to axonal projection patterns in the brain. Testing several other hypotheses will identify specific sensory processing roles for the various interneuron populations in the accessory olfactory bulb. Testing these hypotheses will establish principles of mammalian social odor processing, and will help us to understand the functions that inhibitory neurons provide for sensory neural circuits. Through this research and mentored training plan, Dr. Meeks will produce valuable new data on AOS sensory processing, and will establish new experimental tools and approaches that will form a foundation for a career as an independent scientist.

Public Health Relevance

This research and training plan will provide advanced training in live neuronal imaging and transgenic animal design that will support a career in cutting-edge sensory neuroscience. The innovative approaches used to study distinct populations of interneurons in the mouse accessory olfactory bulb will provide new insight into mammalian olfaction, and will produce greater understanding of sensory processing in the mammalian brain.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Transition Award (R00)
Project #
5R00DC011780-03
Application #
8477017
Study Section
Special Emphasis Panel (NSS)
Program Officer
Sullivan, Susan L
Project Start
2011-07-01
Project End
2015-03-31
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
3
Fiscal Year
2013
Total Cost
$236,102
Indirect Cost
$32,618
Name
University of Texas Sw Medical Center Dallas
Department
Neurosciences
Type
Schools of Medicine
DUNS #
800771545
City
Dallas
State
TX
Country
United States
Zip Code
75390
Hammen, Gary F; Turaga, Diwakar; Holy, Timothy E et al. (2014) Functional organization of glomerular maps in the mouse accessory olfactory bulb. Nat Neurosci 17:953-61
Doyle, Wayne I; Hammen, Gary F; Meeks, Julian P (2014) Ex vivo preparations of the intact vomeronasal organ and accessory olfactory bulb. J Vis Exp :e51813