The aim of the Pathway to Independence Award (K99/R00) is to assist a new investigator in the establishment of an independent research program. In the ROO phase of this grant, the investigator will establish an independent laboratory and will address the remaining aims of the research proposal. The overall goal of the research proposal is to characterize the novel dunc79 gene using the model system Drosophila melanogaster. dunc79 acts as a positive regulator of the NARROW ABDOMEN ion channel, which functions in circadian pacemaker neurons to promote rhythmic behavior. This research will improve our understanding of the neural and genetic basis of circadian rhythmic behaviors. In humans, defects in circadian rhythms are associated with serious conditions such as mental health disorders and metabolic disorders. Moreover, mammalian homologs of the NARROW ABDOMEN ion channel and its regulator DUNC79 are likely to be important for other aspects of health, as mice lacking either gene die shortly after birth. The main specific aims of this project are unmodified from the original proposal. Plans for the ROO phase are indicated.
(Aim 1) To examine the role of dunc79, a putative NA regulator, in Drosophila circadian rhythms. ROO plans include assessing the importance of dunc79 transcript variants to circadian rhythms, and determining whether the loss of dunc79 alters core clock function or expression of the PDF neuropeptide.
(Aim 2) To examine the mechanisms of dunc79 regulation of NA. ROO plans for this aim include assessing and mapping molecular interactions between na and dunc79 .
(Aim 3) To test the hypothesis that dunc79 is circadianly regulated. Plans include assessing potential circadian oscillations of dunc79 transcript and protein.

Public Health Relevance

Defective circadian rhythms in humans are associated with serious conditions such as mental health disorders and metabolic disorders. The fruit fly Drosophila is a proven model system for characterizing genes that regulate circadian rhythms, and many of these genes have similar functions in mammals, including humans.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Transition Award (R00)
Project #
5R00GM080107-05
Application #
8307030
Study Section
Special Emphasis Panel (NSS)
Program Officer
Hagan, Ann A
Project Start
2007-07-30
Project End
2014-07-31
Budget Start
2012-08-01
Budget End
2014-07-31
Support Year
5
Fiscal Year
2012
Total Cost
$246,510
Indirect Cost
$75,735
Name
University of Iowa
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
062761671
City
Iowa City
State
IA
Country
United States
Zip Code
52242