Alzheimer's disease (AD) is a neurodegenerative disease characterized by the accumulation of amyloid plaques in the brain. These plaques are composed of mostly A? peptides generated by proteolysis of the amyloid precursor protein (APP) by two proteases, ?- and ?-secretase. The primary cleavage product is an A? peptide with a length of 40 residues (A?40). However, proteolysis is not highly specific and ~10% of the cleavage products of APP are peptides with two additional amino acids (A?42). The A?42 peptide is more toxic than A?40, and is the principal component of amyloid plaques in the brain. The overarching goal of the proposed research is to establish the mechanism of inhibition for small molecule inhibitors that target neurotoxic A? oligomers in order to design more effective inhibitors. The approach is to combine structural methods with functional assays to determine A?42 structure-function relationships in three specific aims.
The first aim i s to determine the structure and toxicity of the soluble oligomers and fibrils of A?42 using a suite of methods including solution and solid-state nuclear magnetic resonance (NMR) spectroscopy, single touch atomic force microscopy and Fourier transform infrared (FTIR) spectroscopy.
The second aim i s to determine the structure of membrane-bound oligomers and the dynamics of oligomer- membrane interactions. Single molecule total internal reflection fluorescence microscopy will be used to establish the association-dissociation rates and distribution of A?42 bound to membrane bilayers. FTIR spectroscopy will be used to characterize the changes in secondary structure as a function of membrane composition. Solution-state NMR and solid-state NMR spectroscopy will be used to follow specific structural markers identified in Aim 1 that are unique to the oligomers, protofibrils and fibrils.
The third aim i s to determine the mechanism of interaction of small molecule, peptide and protein inhibitors with A? oligomers and fibrils. The small molecule inhibitors include the natural products, curcumin and resveratrol. The peptide inhibitors are designed on the basis of the structure of the A? fibrils. The protein inhibitors are derived from fragments of the myelin basic protein, which we have shown is a natural A? inhibitor in brain white matter. An improved understanding of A?-inhibitor interactions will impact the design of inhibitors to the soluble oligomers. The goal is to establish 1) how the neurotoxic soluble oligomers differ in structure from membrane-bound oligomers and A?42 fibrils, 2) how the addition of two amino acids changes the structure of the A?42 oligomers and fibrils compared to the less toxic A?40 form, and 3) how inhibitors bind to A?42 and prevent toxicity.

Public Health Relevance

Alzheimer's disease is associated with the deposition of fibrillar plaques in the human brain that are composed primarily of a 42-amino acid amyloid-? peptide (A?42). The proposed research is to investigate the molecular structure of A?42 and to develop inhibitors that reduce its toxicity.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Project (R01)
Project #
5R01AG027317-07
Application #
8332308
Study Section
Biophysics of Neural Systems Study Section (BPNS)
Program Officer
Refolo, Lorenzo
Project Start
2006-09-01
Project End
2016-08-31
Budget Start
2012-09-01
Budget End
2013-08-31
Support Year
7
Fiscal Year
2012
Total Cost
$317,935
Indirect Cost
$112,935
Name
State University New York Stony Brook
Department
Biochemistry
Type
Schools of Medicine
DUNS #
804878247
City
Stony Brook
State
NY
Country
United States
Zip Code
11794
Tang, Tzu-Chun; Hu, Yi; Kienlen-Campard, Pascal et al. (2014) Conformational changes induced by the A21G Flemish mutation in the amyloid precursor protein lead to increased A? production. Structure 22:387-96
Xu, Feng; Kotarba, AnnMarie E; Ou-Yang, Ming-Hsuan et al. (2014) Early-onset formation of parenchymal plaque amyloid abrogates cerebral microvascular amyloid accumulation in transgenic mice. J Biol Chem 289:17895-908
Kotarba, Annmarie E; Aucoin, Darryl; Hoos, Michael D et al. (2013) Fine mapping of the amyloid ýý-protein binding site on myelin basic protein. Biochemistry 52:2565-73
Ladiwala, Ali Reza A; Litt, Jeffrey; Kane, Ravi S et al. (2012) Conformational differences between two amyloid ? oligomers of similar size and dissimilar toxicity. J Biol Chem 287:24765-73
Ahmed, Mahiuddin; Davis, Judianne; Aucoin, Darryl et al. (2010) Structural conversion of neurotoxic amyloid-beta(1-42) oligomers to fibrils. Nat Struct Mol Biol 17:561-7
Khalifa, Naouel Ben; Van Hees, Joanne; Tasiaux, Bernadette et al. (2010) What is the role of amyloid precursor protein dimerization? Cell Adh Migr 4:268-72
Liao, Mei-Chen; Hoos, Michael D; Aucoin, Darryl et al. (2010) N-terminal domain of myelin basic protein inhibits amyloid beta-protein fibril assembly. J Biol Chem 285:35590-8
Liao, Mei-Chen; Ahmed, Mahiuddin; Smith, Steven O et al. (2009) Degradation of amyloid beta protein by purified myelin basic protein. J Biol Chem 284:28917-25
Hoos, Michael D; Ahmed, Mahiuddin; Smith, Steven O et al. (2009) Myelin basic protein binds to and inhibits the fibrillar assembly of Abeta42 in vitro. Biochemistry 48:4720-7
Sato, Takeshi; Tang, Tzu-Chun; Reubins, Gabriella et al. (2009) A helix-to-coil transition at the epsilon-cut site in the transmembrane dimer of the amyloid precursor protein is required for proteolysis. Proc Natl Acad Sci U S A 106:1421-6

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