Huntington's disease (HD) is caused by the expansion of a polyglutamine (polyQ) domain in the N-terminal region of the protein huntingtin (htt). While mutant htt is expressed ubiquitously in most types of cells, it leads to selective neurodegeneration that is characterized by severe neuronal loss in the brain striatum of HD patients. Glutamate excitotoxicity has been a long- standing theory to account for the selective degeneration of medium spiny neurons (MSNs) in the striatum. MSNs receive abundant glutamatergic input and are hypersensitive to glutamate stimulation. Since extracellular glutamate is largely removed by astrocytes, a major type of glial cell that supports neuronal survival and protects against neuronal exictotoxicity, any reduction in the ability of astrocytes to remove extracellular glutamate may increase the vulnerability of MSNs to glutamate toxicity. Yet the role of glia in HD neuropathology remains to be fully characterized. Our recent studies show that mutant htt is expressed in astrocytes, forming aggregates in their nuclei. We also found that mutant htt in astrocytes reduces both the expression of the glutamate transporter GLT-1 and the uptake of glutamate. Furthermore, neuron-glia cocultures revealed that normal glia protect against htt-mediated neurotoxicity, whereas glia expressing mutant htt promote neuronal vulnerability to glutamate excitotoxicity. We hypothesize that the expression of mutant htt in glia affects glial function, which leads to altered neurotransmitter uptake and contributes to neuropathology. To test this hypothesis, we will focus on the effects of mutant htt in astrocytes and examine whether mutant htt impedes the important functions of astrocytes in vitro and in vivo.
In Aim 1, we will use cultured primary glial cells to examine how mutant htt affects glutamate uptake and neuronal function in glial cells.
In Aim 2, we will use transgenic mice expressing mutant htt in astrocytes to study how mutant htt in astrocytes contributes to neuronal excitotoxicity.

Public Health Relevance

Huntington disease is caused by a polyglutamine expansion in huntingtin, a protein that is ubiquitously expressed in neuronal and non-neuronal cells. Despite the widespread expression of mutant huntingtin, the disease is characterized by selective degeneration in neurons in the brain. The function and survival of neurons in the brain are largely regulated by glial cells, which are the majority of cells in the brain. However, the role of mutant huntingtin in glial cells and its contribution to neuropathology are unclear. This application will use mouse genetic and molecular neurobiology approaches to investigate whether and how huntingtin in glial cells affects neuronal viability and function. These studies will help develop alternative strategies for treating Huntington disease.

National Institute of Health (NIH)
National Institute on Aging (NIA)
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Clinical Neuroimmunology and Brain Tumors Study Section (CNBT)
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Wise, Bradley C
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Emory University
Schools of Medicine
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Yang, Su; Huang, Shanshan; Gaertig, Marta A et al. (2014) Age-dependent decrease in chaperone activity impairs MANF expression, leading to Purkinje cell degeneration in inducible SCA17 mice. Neuron 81:349-65
Bhat, Kavita P; Yan, Sen; Wang, Chuan-En et al. (2014) Differential ubiquitination and degradation of huntingtin fragments modulated by ubiquitin-protein ligase E3A. Proc Natl Acad Sci U S A 111:5706-11
Wade, Brandy E; Wang, Chuan-En; Yan, Sen et al. (2014) Ubiquitin-activating enzyme activity contributes to differential accumulation of mutant huntingtin in brain and peripheral tissues. J Neurosci 34:8411-22
Weng, Ling; Lin, Yung-Feng; Li, Alina L et al. (2013) Loss of Ahi1 affects early development by impairing BM88/Cend1-mediated neuronal differentiation. J Neurosci 33:8172-84
Xu, Qiaoqiao; Huang, Shanshan; Song, Mingke et al. (2013) Synaptic mutant huntingtin inhibits synapsin-1 phosphorylation and causes neurological symptoms. J Cell Biol 202:1123-38
Cape, Austin; Chen, Xingxing; Wang, Chuan-En et al. (2012) Loss of huntingtin-associated protein 1 impairs insulin secretion from pancreatic *-cells. Cell Mol Life Sci 69:1305-17
Huang, Shanshan; Ling, Joseph J; Yang, Su et al. (2011) Neuronal expression of TATA box-binding protein containing expanded polyglutamine in knock-in mice reduces chaperone protein response by impairing the function of nuclear factor-Y transcription factor. Brain 134:1943-58
Li, Xiao-Jiang; Li, Shihua (2011) Proteasomal dysfunction in aging and Huntington disease. Neurobiol Dis 43:4-8
Havel, Lauren S; Wang, Chuan-En; Wade, Brandy et al. (2011) Preferential accumulation of N-terminal mutant huntingtin in the nuclei of striatal neurons is regulated by phosphorylation. Hum Mol Genet 20:1424-37
Yang, Dongshan; Wang, Chuan-En; Zhao, Bentian et al. (2010) Expression of Huntington's disease protein results in apoptotic neurons in the brains of cloned transgenic pigs. Hum Mol Genet 19:3983-94

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