Continuation of molecular biological, immunological and epitope analysis of toxic shock syndrome toxin 1 is proposed. The unique genetic element associated with tst, the TSST-1 gene, will be mapped and sequenced and tested for transposability. A recently discovered association between tst, trp, and an integrated bla determinant will be characterized at the molecular level. Epitopes involved in mutagenicity and in immune protection will be charcterized and mapped on the TSST-1 molecule and specific and non-specific TSST-1 receptors will be identified and their role in the cytopathology of TSS will be investigated. Finally, tst null mutants of TSS strains isolated from chronic carriers will be used in displacement therapy to eliminate the carrier state.
| Ram, Geeta; Ross, Hope F; Novick, Richard P et al. (2018) Conversion of staphylococcal pathogenicity islands to CRISPR-carrying antibacterial agents that cure infections in mice. Nat Biotechnol 36:971-976 |
| Martínez-Rubio, Roser; Quiles-Puchalt, Nuria; Martí, Miguel et al. (2017) Phage-inducible islands in the Gram-positive cocci. ISME J 11:1029-1042 |
| Novick, Richard P; Ram, Geeta (2017) Staphylococcal pathogenicity islands-movers and shakers in the genomic firmament. Curr Opin Microbiol 38:197-204 |
| Novick, Richard P; Ram, Geeta (2016) The Floating (Pathogenicity) Island: A Genomic Dessert. Trends Genet 32:114-126 |
| Penadés, José R; Chen, John; Quiles-Puchalt, Nuria et al. (2015) Bacteriophage-mediated spread of bacterial virulence genes. Curr Opin Microbiol 23:171-8 |
| Chen, John; Carpena, Nuria; Quiles-Puchalt, Nuria et al. (2015) Intra- and inter-generic transfer of pathogenicity island-encoded virulence genes by cos phages. ISME J 9:1260-3 |
| Chen, John; Ram, Geeta; Penadés, José R et al. (2015) Pathogenicity island-directed transfer of unlinked chromosomal virulence genes. Mol Cell 57:138-49 |
| Chen, John; Ram, Geeta; Yoong, Pauline et al. (2015) An rpsL-based allelic exchange vector for Staphylococcus aureus. Plasmid 79:8-14 |
| Ram, Geeta; Chen, John; Ross, Hope F et al. (2014) Precisely modulated pathogenicity island interference with late phage gene transcription. Proc Natl Acad Sci U S A 111:14536-41 |
| Chen, John; Yoong, Pauline; Ram, Geeta et al. (2014) Single-copy vectors for integration at the SaPI1 attachment site for Staphylococcus aureus. Plasmid 76:1-7 |
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