Chlamydia trachomatis is the leading cause of sexually transmitted diseases (STD) in the developed world. The majority of these infections are subclinical and non-acute in presentation which accounts for the large number of women with tubal infertility and ectopic pregnancies that are so prevalent today. Further, up to 50% of adolescent and young adult women become reinfected with Chlamydia which contributes to tubal scarring. Thus, adolescents are an important target group in which to analyze transmission patterns and types of recurrence in order to design appropriate interventional strategies. Strain typing is essential for defining the molecular epidemiology of chlamydial infections within the context of different chlamydial asymptomatic and symptomatic disease states that include urethritis, cervicitis, pelvic inflammatory disease, and recurrent infection. Monoclonal antibodies to the major outer membrane protein (MOMP) of C. trachomatis have historically been used for typing. Yet, serotypes do not reflect the same degree of variation found at the gene and protein level of this organism. ompl genotyping has been developed which is based on the MOMP gene (ompl ). It is capable of detecting new subtypes of currently known serotypes and provides a powerful alternative to immunotyping. Genotyping is an appropriate tool for evaluating transmission patterns and recurrent infections, and for use as a biologic measure of behavior in behavioral related studies and for public health surveillance.
Specific Aims : 1) define the ompl genotypes and variants among adolescent males and females who differ by force-of-infection and behavioral variables; 2) analyze transmission patterns of ompl genotypes between partners and within teen groups; 3) evaluate types of recurrent infections that occur in adolescents in comparison with first (naive) or previous infections, define types of mutations that occur in ompl over these time points, and evaluate the role of genotype polymorphism, co-pathogens, and immune response in reinfection at cervical and urethral sites; and 4) define molecular characteristics of genotypes that may be associated with clinical and microbiologic data; analyze the sequence-structure/function relationships of MOMP from prototype and variant strains.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI039499-02
Application #
2672709
Study Section
Bacteriology and Mycology Subcommittee 2 (BM)
Project Start
1997-06-01
Project End
2001-05-31
Budget Start
1998-06-01
Budget End
1999-05-31
Support Year
2
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of California San Francisco
Department
Ophthalmology
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143
Sharma, Manu; Recuero-Checa, Maria A; Fan, Frances Yue et al. (2018) Chlamydia trachomatis regulates growth and development in response to host cell fatty acid availability in the absence of lipid droplets. Cell Microbiol 20:
Somboonna, Naraporn; Wan, Raymond; Ojcius, David M et al. (2011) Hypervirulent Chlamydia trachomatis clinical strain is a recombinant between lymphogranuloma venereum (L(2)) and D lineages. MBio 2:e00045-11
Atik, Berna; Johnston, S Claiborne; Dean, Deborah (2010) Association of carotid plaque Lp-PLA(2) with macrophages and Chlamydia pneumoniae infection among patients at risk for stroke. PLoS One 5:e11026
Dean, Deborah; Bruno, William J; Wan, Raymond et al. (2009) Predicting phenotype and emerging strains among Chlamydia trachomatis infections. Emerg Infect Dis 15:1385-94
Tan, Chun; Hsia, Ru-ching; Shou, Huizhong et al. (2009) Chlamydia trachomatis-infected patients display variable antibody profiles against the nine-member polymorphic membrane protein family. Infect Immun 77:3218-26
Somboonna, Naraporn; Mead, Sally; Liu, Jessica et al. (2008) Discovering and differentiating new and emerging clonal populations of Chlamydia trachomatis with a novel shotgun cell culture harvest assay. Emerg Infect Dis 14:445-53
Skwor, Troy A; Atik, Berna; Kandel, Raj Prasad et al. (2008) Role of secreted conjunctival mucosal cytokine and chemokine proteins in different stages of trachomatous disease. PLoS Negl Trop Dis 2:e264
Nunes, Alexandra; Gomes, Joao P; Mead, Sally et al. (2007) Comparative expression profiling of the Chlamydia trachomatis pmp gene family for clinical and reference strains. PLoS One 2:e878
Gomes, Joao P; Bruno, William J; Nunes, Alexandra et al. (2007) Evolution of Chlamydia trachomatis diversity occurs by widespread interstrain recombination involving hotspots. Genome Res 17:50-60
Gomes, Joao P; Borrego, Maria J; Atik, Berna et al. (2006) Correlating Chlamydia trachomatis infectious load with urogenital ecological success and disease pathogenesis. Microbes Infect 8:16-26

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