For replication of influenza virus, 3 viral polymerase (3P) proteins (PA, PB1 and PB2) are necessary. The long term goal of this proposal is to find ways to interfere with the polymerase activity for a safe live virus vaccine.
The aims of this proposal are: 1. how the polymerase complex is formed, 2. what are the functions of each polymerase protein, 3. what are the domains and sequence requirements for these functions, 4. what are the functions of nucleocapsid protein (NP) in modulating the polymerase activity, and 5. what are the requirements of type (A, B, and C) specificity for viral polymerases?

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
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Virology Study Section (VR)
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University of California Los Angeles
Schools of Medicine
Los Angeles
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Nayak, Debi P; Balogun, Rilwan A; Yamada, Hiroshi et al. (2009) Influenza virus morphogenesis and budding. Virus Res 143:147-61
Barman, Subrata; Nayak, Debi P (2007) Lipid raft disruption by cholesterol depletion enhances influenza A virus budding from MDCK cells. J Virol 81:12169-78
Hui, Eric Ka-Wai; Smee, Donald F; Wong, Min-Hui et al. (2006) Mutations in influenza virus M1 CCHH, the putative zinc finger motif, cause attenuation in mice and protect mice against lethal influenza virus infection. J Virol 80:5697-707
Hui, Eric Ka-Wai; Barman, Subrata; Tang, Dominic Ho-Ping et al. (2006) YRKL sequence of influenza virus M1 functions as the L domain motif and interacts with VPS28 and Cdc42. J Virol 80:2291-308
Hui, Eric Ka-Wai; Yap, Ee Ming; An, Dong Sung et al. (2004) Inhibition of influenza virus matrix (M1) protein expression and virus replication by U6 promoter-driven and lentivirus-mediated delivery of siRNA. J Gen Virol 85:1877-84
Nayak, Debi P; Hui, Eric Ka-Wai; Barman, Subrata (2004) Assembly and budding of influenza virus. Virus Res 106:147-65
Nayak, Debi P; Hui, Eric K W (2004) The role of lipid microdomains in virus biology. Subcell Biochem 37:443-91
Barman, Subrata; Adhikary, Lopa; Chakrabarti, Alok K et al. (2004) Role of transmembrane domain and cytoplasmic tail amino acid sequences of influenza a virus neuraminidase in raft association and virus budding. J Virol 78:5258-69
Hui, Eric Ka-Wai; Ralston, Katherine; Judd, Amrit K et al. (2003) Conserved cysteine and histidine residues in the putative zinc finger motif of the influenza A virus M1 protein are not critical for influenza virus replication. J Gen Virol 84:3105-13
Barman, Subrata; Adhikary, Lopa; Kawaoka, Yoshihiro et al. (2003) Influenza A virus hemagglutinin containing basolateral localization signal does not alter the apical budding of a recombinant influenza A virus in polarized MDCK cells. Virology 305:138-52

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