Abstract

To determine the optimal combination of enhancer and boundary elements for use in mosquito transgenesis, the following specific aims are to be completed: 1) evaluate a number of enhancer elements to determine which one causes the largest increase in transcription levels in ae. aegypti; 2) assess the ability of transcriptional activator proteins to further increase the effects of enhancer elements; 30 determine if boundary elements can protect transgenes in Ae. aegypti from position effects; 4) determine if the addition of enhancer and boundary elements can improve the levels of expression from tissue-specific promoters.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
1R01AI046432-01
Application #
6028109
Study Section
Special Emphasis Panel (ZRG1-TMP (01))
Program Officer
Aultman, Kathryn S
Project Start
2000-02-01
Project End
2005-01-31
Budget Start
2000-02-01
Budget End
2001-01-31
Support Year
1
Fiscal Year
2000
Total Cost
$230,483
Indirect Cost

  Institution

Name
Texas A&M University
Department
Zoology
Type
Schools of Earth Sciences/Natur
DUNS #
047006379
City
College Station
State
TX
Country
United States
Zip Code
77845

  Publications

Gray, Christine E; Coates, Craig J (2005) Cloning and characterization of cDNAs encoding putative CTCFs in the mosquitoes, Aedes aegypti and Anopheles gambiae. BMC Mol Biol 6:16
Gray, Christine E; Coates, Craig J (2004) High-level gene expression in Aedes albopictus cells using a baculovirus Hr3 enhancer and IE1 transactivator. BMC Mol Biol 5:8
Tu, Zhijian; Coates, Craig (2004) Mosquito transposable elements. Insect Biochem Mol Biol 34:631-44