There were two types of setbacks to this R01 project. Lab staff lost 2 months of work as will be explained and material were destroyed in cold rooms and some refrigerators that failed due to insufficient emergency power early in the outage. The full closure of the Skirball Institute for 2 weeks caused a disruption of work that resulted in loss of experiments started up on 2 weeks prior to the storm on Oct 29 and required an additional month to fully recover cell cultures and reagents to resume experiments.

Public Health Relevance

Vaccination strategies depend upon a physical embrace between antigen specific T lymphocytes and antigen presenting dendritic cells. We hypothesize that the stability of this embrace will depend upon the quality of antigen that defines a stop signal for the migrating T cell; signals in the environment that provide a competing go signal to the lymphocyte and polarity networks controlled by kinases. We propose experiments to determine the roles of chemokines; dendritic cell frequency and regulatory T cell protein kinase C-8 in tolerance induction.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Project (R01)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1 (02))
Program Officer
Lapham, Cheryl K
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
New York University
Schools of Medicine
New York
United States
Zip Code
Coles, Jonathan A; Stewart-Hutchinson, Phillip J; Myburgh, Elmarie et al. (2017) The mouse cortical meninges are the site of immune responses to many different pathogens, and are accessible to intravital imaging. Methods 127:53-61
Jang, Jung-Eun; Hajdu, Cristina H; Liot, Caroline et al. (2017) Crosstalk between Regulatory T Cells and Tumor-Associated Dendritic Cells Negates Anti-tumor Immunity in Pancreatic Cancer. Cell Rep 20:558-571
Shaw, Tovah N; Stewart-Hutchinson, Phillip J; Strangward, Patrick et al. (2015) Perivascular Arrest of CD8+ T Cells Is a Signature of Experimental Cerebral Malaria. PLoS Pathog 11:e1005210
Mayya, Viveka; Neiswanger, Willie; Medina, Ricardo et al. (2015) Integrative analysis of T cell motility from multi-channel microscopy data using TIAM. J Immunol Methods 416:84-93
Kuan, Emma L; Ivanov, Stoyan; Bridenbaugh, Eric A et al. (2015) Collecting lymphatic vessel permeability facilitates adipose tissue inflammation and distribution of antigen to lymph node-homing adipose tissue dendritic cells. J Immunol 194:5200-10
Choudhuri, Kaushik; LlodrĂ¡, Jaime; Roth, Eric W et al. (2014) Polarized release of T-cell-receptor-enriched microvesicles at the immunological synapse. Nature 507:118-23
Dustin, Michael L (2014) The immunological synapse. Cancer Immunol Res 2:1023-33
Williams, Cassandra R; Dustin, Michael L; Sauer, John-Demian (2013) Inflammasome-mediated inhibition of Listeria monocytogenes-stimulated immunity is independent of myelomonocytic function. PLoS One 8:e83191
Waite, Janelle C; Vardhana, Santosh; Shaw, Patrick J et al. (2013) Interference with Ca(2+) release activated Ca(2+) (CRAC) channel function delays T-cell arrest in vivo. Eur J Immunol 43:3343-54
Ruocco, Maria Grazia; Pilones, Karsten A; Kawashima, Noriko et al. (2012) Suppressing T cell motility induced by anti-CTLA-4 monotherapy improves antitumor effects. J Clin Invest 122:3718-30

Showing the most recent 10 out of 23 publications