There were two types of setbacks to this R01 project. Lab staff lost 2 months of work as will be explained and material were destroyed in cold rooms and some refrigerators that failed due to insufficient emergency power early in the outage. The full closure of the Skirball Institute for 2 weeks caused a disruption of work that resulted in loss of experiments started up on 2 weeks prior to the storm on Oct 29 and required an additional month to fully recover cell cultures and reagents to resume experiments.
Vaccination strategies depend upon a physical embrace between antigen specific T lymphocytes and antigen presenting dendritic cells. We hypothesize that the stability of this embrace will depend upon the quality of antigen that defines a stop signal for the migrating T cell; signals in the environment that provide a competing go signal to the lymphocyte and polarity networks controlled by kinases. We propose experiments to determine the roles of chemokines; dendritic cell frequency and regulatory T cell protein kinase C-8 in tolerance induction.
|Waite, Janelle C; Vardhana, Santosh; Shaw, Patrick J et al. (2013) Interference with Ca(2+) release activated Ca(2+) (CRAC) channel function delays T-cell arrest in vivo. Eur J Immunol 43:3343-54|
|Schwickert, Tanja A; Victora, Gabriel D; Fooksman, David R et al. (2011) A dynamic T cell-limited checkpoint regulates affinity-dependent B cell entry into the germinal center. J Exp Med 208:1243-52|
|Kim, Jiyun V; Jiang, Ning; Tadokoro, Carlos E et al. (2010) Two-photon laser scanning microscopy imaging of intact spinal cord and cerebral cortex reveals requirement for CXCR6 and neuroinflammation in immune cell infiltration of cortical injury sites. J Immunol Methods 352:89-100|
|Fooksman, David R; Schwickert, Tanja A; Victora, Gabriel D et al. (2010) Development and migration of plasma cells in the mouse lymph node. Immunity 33:118-27|
|Fooksman, David R; Vardhana, Santosh; Vasiliver-Shamis, Gaia et al. (2010) Functional anatomy of T cell activation and synapse formation. Annu Rev Immunol 28:79-105|
|Zanin-Zhorov, Alexandra; Ding, Yi; Kumari, Sudha et al. (2010) Protein kinase C-theta mediates negative feedback on regulatory T cell function. Science 328:372-6|
|Kim, Jiyun V; Kang, Silvia S; Dustin, Michael L et al. (2009) Myelomonocytic cell recruitment causes fatal CNS vascular injury during acute viral meningitis. Nature 457:191-5|
|Velazquez, Peter; Cameron, Thomas O; Kinjo, Yuki et al. (2008) Cutting edge: activation by innate cytokines or microbial antigens can cause arrest of natural killer T cell patrolling of liver sinusoids. J Immunol 180:2024-8|
|Dustin, Michael L; Chakraborty, Arup K (2008) Tug of war at the exit door. Immunity 28:15-7|
|Matsumura, Satoko; Wang, Baomei; Kawashima, Noriko et al. (2008) Radiation-induced CXCL16 release by breast cancer cells attracts effector T cells. J Immunol 181:3099-107|
Showing the most recent 10 out of 12 publications