There were two types of setbacks to this R01 project. Lab staff lost 2 months of work as will be explained and material were destroyed in cold rooms and some refrigerators that failed due to insufficient emergency power early in the outage. The full closure of the Skirball Institute for 2 weeks caused a disruption of work that resulted in loss of experiments started up on 2 weeks prior to the storm on Oct 29 and required an additional month to fully recover cell cultures and reagents to resume experiments.
Vaccination strategies depend upon a physical embrace between antigen specific T lymphocytes and antigen presenting dendritic cells. We hypothesize that the stability of this embrace will depend upon the quality of antigen that defines a stop signal for the migrating T cell; signals in the environment that provide a competing go signal to the lymphocyte and polarity networks controlled by kinases. We propose experiments to determine the roles of chemokines; dendritic cell frequency and regulatory T cell protein kinase C-8 in tolerance induction.
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