The on-going West Nile virus (WNV) epidemic is the largest mosquito-borne outbreak in California and North American history and the largest WNV outbreak documented globally. WNV has now become endemic throughout California providing a unique natural experiment to determine how this invading virus will persist within different mosquito - avian transmission cycles over varying landscapes, ranging from the southeastern deserts to densely populated maritime Los Angeles to agricultural and rural settings throughout the Central Valley. Our competitive continuation proposal investigates the cascade of early season epidemiological events that determine whether WNV will remain at low persistence levels or amplify to outbreak levels. Quantitative delineation of these events and their precursors may enhance decision support systems and allow skillful forecasting to trigger timely and effective intervention. Concurrent with the WNV invasion has been the elimination of St. Louis encephalitis virus (SLEV), a previously endemic and closely related virus within the Japanese encephalitis virus (JEV) serocomplex. Since the level of WNV transmission presumably dictates the receptivity of California for the re-establishment of SLEV, our planned research also will address our previous hypothesis that similarity in natural history among closely related flaviviruses within the JEV serocomplex precludes concurrent sympatric amplification. We propose to address our hypotheses with the following specific aims: 1) Determine the relative importance of different mechanism[s] of WNV overwintering in different biomes, including continued horizontal transmission at southern latitudes, vertical transmission within mosquito populations, and relapse of chronic avian infection. 2) Delineate the timing of Culex spp. diapause termination, the onset of blood feeding and the initiation of enzootic transmission;and 3) evaluate the impact of vernal avian seroprevalence ['herd immunity'] on WNV amplification and SLEV introduction. Our new research will provide data on whether WNV has overwintered successfully, degree-day functions to anticipate the initiation of virus amplification, and the impact of herd immunity on the rate and level of amplification. Combining these factors into an early season risk models may allow us to forecast outbreaks of WNV in California and perhaps western North America.

Public Health Relevance

West Nile virus (WNV), a serious public and veterinary health problem throughout the North America, is now endemic throughout California, but it remains unclear what epidemiological factors will allow it to persist and resurge to outbreak levels. Our competitive continuation proposal investigates the cascade of early season events that determine if WNV will remain at low maintenance levels or amplify to create outbreaks. Quantitative delineation of these events and their precursors may allow skillful forecasting and enhance decision support systems to trigger timely intervention.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
4R01AI055607-08
Application #
8201668
Study Section
Vector Biology Study Section (VB)
Program Officer
Repik, Patricia M
Project Start
2003-07-01
Project End
2013-05-31
Budget Start
2011-06-14
Budget End
2012-05-31
Support Year
8
Fiscal Year
2011
Total Cost
$554,251
Indirect Cost
Name
University of California Davis
Department
Public Health & Prev Medicine
Type
Schools of Veterinary Medicine
DUNS #
047120084
City
Davis
State
CA
Country
United States
Zip Code
95618
Brault, Aaron C; Fang, Ying; Reisen, William K (2015) Multiplex qRT-PCR for the Detection of Western Equine Encephalomyelitis, St. Louis Encephalitis, and West Nile Viral RNA in Mosquito Pools (Diptera: Culicidae). J Med Entomol 52:491-9
Duggal, Nisha K; Reisen, William K; Fang, Ying et al. (2015) Genotype-specific variation in West Nile virus dispersal in California. Virology 485:79-85
Worwa, Gabriella; Wheeler, Sarah S; Brault, Aaron C et al. (2015) Comparing competitive fitness of West Nile virus strains in avian and mosquito hosts. PLoS One 10:e0125668
Hinton, M G; Reisen, W K; Wheeler, S S et al. (2015) West Nile Virus Activity in a Winter Roost of American Crows (Corvus brachyrhynchos): Is Bird-To-Bird Transmission Important in Persistence and Amplification? J Med Entomol 52:683-92
Maharaj, Payal D; Bolling, Bethany G; Anishchenko, Michael et al. (2014) Genetic determinants of differential oral infection phenotypes of West Nile and St. Louis encephalitis viruses in Culex spp. mosquitoes. Am J Trop Med Hyg 91:1066-72
Reisen, William K (2014) Medical entomology--back to the future? Infect Genet Evol 28:573-82
Langevin, Stanley A; Bowen, Richard A; Reisen, William K et al. (2014) Host competence and helicase activity differences exhibited by West Nile viral variants expressing NS3-249 amino acid polymorphisms. PLoS One 9:e100802
Worwa, Gabriella; Andrade, Christy C; Thiemann, Tara C et al. (2014) Allele-specific qRT-PCR demonstrates superior detection of single nucleotide polymorphisms as genetic markers for West Nile virus compared to Luminex® and quantitative sequencing. J Virol Methods 195:76-85
Barker, Christopher M; Niu, Tianchan; Reisen, William K et al. (2013) Data-driven modeling to assess receptivity for Rift Valley Fever virus. PLoS Negl Trop Dis 7:e2515
Reisen, William K; Padgett, Kerry; Fang, Ying et al. (2013) Chronic infections of West Nile virus detected in California dead birds. Vector Borne Zoonotic Dis 13:401-5

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