Immune suppression of self-reactive CD4+ and CD8+ T cells by natural, thymic-derived CD4+CD25+ T regulatory cells is crucial for the maintenance of peripheral self-tolerance. Recent data suggest that in addition to anti-self natural Treg cells, a population of Treg cells, activated in the peripheral lymphoid tissues by pathogens may play a major role in controlling excessive """"""""inflammatory"""""""" responses to infection. Natural and pathogen-induced Treg cells are phenotypically and functionally similar in that they express CD25, GITR, and the transcriptional regulatory protein Foxp3, and are immunosuppressive for antigen-activated T cells, suggesting that natural and peripheral activated Treg cells may be of the same lineage. However, we have recently demonstrated in the FIV AIDS lentiviruses infection that pathogen-induced Treg cells differ from natural Treg cells in that they express TGF-2 on their surface (mTGF-2+) and that mTGF-2 mediates Treg suppressor function and may also control peripheral Treg homeostasis. The experiments proposed herein will further explore these observations to test the hypothesis that Treg cells in the peripheral immune tissues are a normal component of the immune regulatory process to infectious agents and that AIDS lentiviruses over-ride the normal controls on Treg activation and function, resulting in chronic immunosuppressive activity and abnormal Treg homeostasis. CD4+CD25+ Treg cells will be assessed phenotypically (mTGF-2+, Foxp3+) and functionally (inhibition of ConA-induced proliferation and IL2 by CD4+ Th cells and IFN-3 by CD8+ T cells) in cats acutely infected with the NCSU1 isolate of FIV. FIV gag ELISA and RT-PCR assays will be performed to determine if Treg activation is associated with virus infection. The role of mTGF-2 in Treg suppressor function will be determined by the use of TGF-2 and TGF-2RII neutralizing antibodies. To investigate the role of mTGF-2 in maintaining peripheral Treg homeostasis in FIV+ cats by recruitment from the CD4+ Th pool, we will incubate mTGF-2+ Treg cells with CD4+ Th cells and analyze the target cells for expression of CD25, Foxp3, mTGF-2 and for suppressor function. To confirm the mTGF-2+ Treg cells induced conversion of Th cells to Treg cells, we will utilize TGF-2 and TGF-2RII neutralizing antibodies to block the conversion process. These studies will address two important immunological issues: 1) how do CD4+CD25+ Treg cells mediate suppression and phenotypic conversion of CD4+ and/or CD8+ Th populations to maintain their homeostasis;and 2) how do AIDS lentivirus infections over-ride the normal controls over Treg cell activation and function, resulting in chronic Treg-induced immunosuppression, as well as abnormal Treg homeostasis.

Public Health Relevance

T regulatory (Treg) cells play a pivotal role in maintaining the balance between protective immune responses and immunopathology associated with primary infections. We believe that AIDS lentivirus infections such as HIV over-ride the normal controls over Treg cell activation and function, resulting in chronic Treg cell activation and global immunosuppression and AIDS. Understanding how HIV co-opts this normal immune regulatory mechanism, which will be addressed in this proposal, will aid in the development of better therapeutic modalities.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI080288-02
Application #
7739491
Study Section
AIDS Immunology and Pathogenesis Study Section (AIP)
Program Officer
Embry, Alan C
Project Start
2008-12-01
Project End
2012-11-30
Budget Start
2009-12-01
Budget End
2010-11-30
Support Year
2
Fiscal Year
2010
Total Cost
$368,775
Indirect Cost
Name
North Carolina State University Raleigh
Department
Veterinary Sciences
Type
Schools of Veterinary Medicine
DUNS #
042092122
City
Raleigh
State
NC
Country
United States
Zip Code
27695
Miller, Michelle M; Akaronu, Nnenna; Thompson, Elizabeth M et al. (2015) Modulating DNA methylation in activated CD8+ T cells inhibits regulatory T cell-induced binding of Foxp3 to the CD8+ T Cell IL-2 promoter. J Immunol 194:990-8
Meng, Liping; Tompkins, Mary; Miller, Michelle et al. (2014) Lentivirus-activated T regulatory cells suppress T helper cell interleukin-2 production by inhibiting nuclear factor of activated T cells 2 binding to the interleukin-2 promoter. AIDS Res Hum Retroviruses 30:58-66
Miller, Michelle M; Petty, Christopher S; Tompkins, Mary B et al. (2014) CD4+CD25+ T regulatory cells activated during feline immunodeficiency virus infection convert T helper cells into functional suppressors through a membrane-bound TGF? / GARP-mediated mechanism. Virol J 11:7
Miller, Michelle M; Thompson, Elizabeth M; Suter, Steven E et al. (2013) CD8+ clonality is associated with prolonged acute plasma viremia and altered mRNA cytokine profiles during the course of feline immunodeficiency virus infection. Vet Immunol Immunopathol 152:200-8
Fogle, J E; Tompkins, W A; Campbell, B et al. (2011) Fozivudine tidoxil as single-agent therapy decreases plasma and cell-associated viremia during acute feline immunodeficiency virus infection. J Vet Intern Med 25:413-8
Fogle, Jonathan E; Mexas, Angela M; Tompkins, Wayne A et al. (2010) CD4(+)CD25(+) T regulatory cells inhibit CD8(+) IFN-gamma production during acute and chronic FIV infection utilizing a membrane TGF-beta-dependent mechanism. AIDS Res Hum Retroviruses 26:201-16
Fogle, Jonathan E; Tompkins, Wayne A; Tompkins, Mary B (2010) CD4+CD25+ T regulatory cells from FIV+ cats induce a unique anergic profile in CD8+ lymphocyte targets. Retrovirology 7:97