We have identified more than 40,000 single nucleotide polymorphisms (SNPs) in 16 samples of Aedes aegypti from around the world, as well as a sample of its closest relative, Ae. mascarensis. This was done using a technique that is efficient in identifying SNPs (RAD-tags), but this methodology is not applicable for routine genotyping. We propose to develop a SNP chip based on these identified SNPs that can be applied routinely for reliable genotyping in a time- and cost-effective manner. Our own interests are on the population genetics and ancestry level and we will focus on a chip designed specifically for our studies. A by-product of the work will be more extensive data upon which other groups could design a chip for genome wide association studies;all data will be publically available. The first part of the project is development of the population chip. This will proceed i three steps: (1) From the >40,000 available SNPs, identify ~5,000 that have the most variation across the species'distribution and reliably genotype on SNP chips. (2) From these 5,000, use theoretical analyses to identify ~750 that capture the population/ancestry information of the complete data set. (2) Perform crosses to confirm the Mendelian (single-copy, nuclear) nature of these 750, with the expectation that at least 500 will be Mendelian and form the basis of the population SNP chip. The second part of the project is application of this chip. Four projects are proposed: (1) Perform a global population genetics study to determine the genetic diversity, patterns, and relatedness of populations from around the world;collections are already in hand for this. (2) Study five ecologically and geographically diverse populations through time to determine the genetic stability of Ae. aegypti populations as well as effective population sizes. (3) Genotype common lab strains to determine where they fit into the genetic diversity of the species and how homogeneous or heterogeneous they are among isolates from different laboratories. (4) Genotype populations in West Africa that have recently colonized urban centers and compare them to nearby sylvan populations that are the likely source of the """"""""domestication"""""""" event;independent replicate cases will allow us to identify parallel genetic changes that would give insight into the genetic nature of the domestication process.

Public Health Relevance

Aedes aegypti, the yellow fever mosquito, is more feared today as the major vector of dengue fever, a viral disease that threatens fully 40% of the global population. We propose to use the most up-to-date technologies to genetically characterize this mosquito that exhibits extremely high diversity in terms of ecology (breeding in forests, towns, and urban centers) and behavior (e.g., biting animals in some localities, humans in others).

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Project (R01)
Project #
Application #
Study Section
Vector Biology Study Section (VB)
Program Officer
Costero-Saint Denis, Adriana
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Yale University
Schools of Arts and Sciences
New Haven
United States
Zip Code
IbaƱez-Justicia, A; Gloria-Soria, A; den Hartog, W et al. (2017) The first detected airline introductions of yellow fever mosquitoes (Aedes aegypti) to Europe, at Schiphol International airport, the Netherlands. Parasit Vectors 10:603
Saarman, Norah P; Gloria-Soria, Andrea; Anderson, Eric C et al. (2017) Effective population sizes of a major vector of human diseases, Aedes aegypti. Evol Appl 10:1031-1039
Gloria-Soria, A; Kellner, D A; Brown, J E et al. (2016) Temporal genetic stability of Stegomyia aegypti (= Aedes aegypti) populations. Med Vet Entomol 30:235-40
Gloria-Soria, Andrea; Ayala, Diego; Bheecarry, Ambicadutt et al. (2016) Global genetic diversity of Aedes aegypti. Mol Ecol 25:5377-5395
Powell, Jeffrey R; Dion, Kirstin (2015) Effects of codon usage on gene expression: empirical studies on Drosophila. J Mol Evol 80:219-26
Richardson, Joshua B; Jameson, Samuel B; Gloria-Soria, Andrea et al. (2015) Evidence of limited polyandry in a natural population of Aedes aegypti. Am J Trop Med Hyg 93:189-93
Evans, Benjamin R; Gloria-Soria, Andrea; Hou, Lin et al. (2015) A Multipurpose, High-Throughput Single-Nucleotide Polymorphism Chip for the Dengue and Yellow Fever Mosquito, Aedes aegypti. G3 (Bethesda) 5:711-8
Powell, Jeffrey R; Besansky, Nora J; della Torre, Alessandra et al. (2014) Mario Coluzzi (1938-2012). Malar J 13:10
Brown, Julia E; Evans, Benjamin R; Zheng, Wei et al. (2014) Human impacts have shaped historical and recent evolution in Aedes aegypti, the dengue and yellow fever mosquito. Evolution 68:514-25
Walter, Katharine S; Brown, Julia E; Powell, Jeffrey R (2014) Microhabitat partitioning of Aedes simpsoni (Diptera: Culicidae). J Med Entomol 51:596-604

Showing the most recent 10 out of 18 publications