Our studies using mumps virus (MuV), a paramyxovirus, will unveil the mechanism by which the viral RdRp recognizes the nucleocapsid and gains access to the viral genomic RNA sequestered inside the nucleocapsid. It will also address how the rule of six is imposed by the nucleocapsid and how mRNA editing is regulated by interactions between P and N.
Aim 1. The molecular mechanism for P functions. Our preliminary studies have shown that MuV P forms a tetramer with one pair of two parallel subunits, and another pair in the opposite orientation. This orientation that places the N-terminal and C-terminal regions on both ends of the MuV P tetramer is a novel structure moiety of P. Our data also showed that both N- and C-terminal regions are involved in binding specifically to the nucleocapsid, unlike P proteins of other NSVs that requires only the C- terminal region.
In aim 1 a, we will determine the crystal structure of the N-terminal domains and the C-terminal domains of MuV P. Crystal hits have been observed.
In aim 1 b, certain regions of P may be truncated and their effects on viral transcription and replication will be examined using a mini-genome system and a reverse genetics system.
In aim 1 c, specific mutational analysis based on the crystal structure of the N-terminal domain, the oligomerization domain and the C-terminal domain of MuV P will be carried out. For these mutants, interactions with the N proteins will be examined and their effects on viral transcription and replication will also be examined using a mini-genome system and a reverse genetics system. Alternative approaches include H/D exchange by mass spectrometry to map protein interactions.
Aim 2. The molecular mechanism for N functions. We have previously prepared a nucleocapsid-like particle (NLP) that contains 13 N subunits and a piece of random RNA. This NLP corresponds to one turn of the helical nucleocapsid of MuV. MuV P and its nucleocapsid binding domains (both at N- and C- terminal regions) were shown to bind NLP. Proteolytic removal of the C-terminal region at residue 379 did not disrupt NLP or P binding.
In aim 2 a, the three dimensional structure of the NLP or its truncated version (N379) will be solved by X-ray crystallography. Crystals of NLP have been grown.
In aim 2 b, the location of P interactions with MuV NLP will be determined. We will solve the cryoEM structure of P or P fragments in complex with NLP or truncated NLP. When possible, P fragments may be cocrystallized with NLP or truncated NLP and the respective structure will be solved by X- ray crystallography. H/D exchange by mass spectrometry will be an alternative approach.
In aim 2 c, mutations will be generated to alter interactions between N and P, and their effects on NLP assembly and protein binding will be examined. Effects of mutations on viral transcription and replication will also be examined in a mini- genome system and a reverse genetics system.

Public Health Relevance

Paramyxoviruses are important human pathogens. The mechanism of their replications are different from the rest of viruses, such as the 'rule of six' that stipulates the genome sequence to be read in blocks of six nucleotides. The experiments in this application are designed to unveil the unique mechanism of paramyxovirus replication.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI106307-04
Application #
9114385
Study Section
Special Emphasis Panel (ZRG1-IDM-N (02)S)
Program Officer
Challberg, Mark D
Project Start
2013-08-01
Project End
2017-07-31
Budget Start
2016-08-01
Budget End
2017-07-31
Support Year
4
Fiscal Year
2016
Total Cost
$571,779
Indirect Cost
$129,081
Name
Georgia State University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
837322494
City
Atlanta
State
GA
Country
United States
Zip Code
30302
Pickar, Adrian; Zengel, James; Xu, Pei et al. (2016) Mumps Virus Nucleoprotein Enhances Phosphorylation of the Phosphoprotein by Polo-Like Kinase 1. J Virol 90:1588-98
Pickar, Adrian; Elson, Andrew; Yang, Yang et al. (2015) Oligomerization of Mumps Virus Phosphoprotein. J Virol 89:11002-10
Zengel, James; Pickar, Adrian; Xu, Pei et al. (2015) Roles of Phosphorylation of the Nucleocapsid Protein of Mumps Virus in Regulating Viral RNA Transcription and Replication. J Virol 89:7338-47
Cox, Robert; Pickar, Adrian; Qiu, Shihong et al. (2014) Structural studies on the authentic mumps virus nucleocapsid showing uncoiling by the phosphoprotein. Proc Natl Acad Sci U S A 111:15208-13
Green, Todd J; Cox, Robert; Tsao, Jun et al. (2014) Common mechanism for RNA encapsidation by negative-strand RNA viruses. J Virol 88:3766-75
Pickar, Adrian; Xu, Pei; Elson, Andrew et al. (2014) Roles of serine and threonine residues of mumps virus P protein in viral transcription and replication. J Virol 88:4414-22