About 43 million individuals in the US currently suffer from disabilities due to arthritis. Cartilage defects are the major source of pain in the affected joints. Current treatments, whilst alleviating some of the clinical symptoms, prove insufficient to cure the underlying irreversible cartilage loss. Stem cells represent a unique source for restoration of cartilage defects. However, a major challenge with various approaches of stem cell and chondrocyte transplants is death of the transplanted cells with clearance by the immune system. The overall goal of this project is to develop a non-invasive and clinically applicable MR imaging technique, which provides an early diagnosis of complications of the engraftment process of matrix associated stem cell implants (MASI). The hypothesis is that viable and apoptotic MASI in arthritic joints demonstrate distinct signal characteristics on MR images, when combined with cell-specific MR contrast agents. In our current funding period, we have established labeling techniques for stem cell transplants with iron oxide nanoparticles and gadolinium chelates, and we have defined distinct signal characteristics of labeled viable and apoptotic MASI. During the new funding period, we will continue to use this approach to identify and develop strong candidate contrast agents for clinical translation. We plan to apply for an IND for clinical translation of the most promising approach by the end of this new funding period. In our pursuit of clinically applicable imaging approaches, we will first evaluate stem cell loss and/or apoptosis early after MASI via direct labeling of transplanted stem cells with the FDA-approved iron oxide nanoparticle ferumoxytol (Feraheme). In a second step, we will evaluate a novel caspase-sensitive MR contrast agent regarding its sensitivity to detect stem cell apoptosis in MASI with an arthrographic imaging approach. In a third step, we will identify failed MASI indirectly, via MR detection of the migration of iron oxide labeled bone marrow macrophages into MASI. Complementary optical imaging studies as well as confocal microscopy, immunohistochemistry and spectrometry studies will be performed to elucidate biological and physicochemical changes of the investigated cell transplants that lead to the observed MR signal characteristics. Results should be immediately helpful in the preclinical assessment of new stem cell based therapies for arthritis treatment, in the design of related clinical trials for hMSC therapy of arthritis, and ultimately, in the assessment of those hMSC therapy regimens in clinical practice. By exploiting novel, clinically applicable cell tracking techniques as a new too to monitor stem cell engraftment outcomes non-invasively in vivo, we anticipate significantly improving and accelerating the development of successful therapies for cartilage regeneration in patients, and ultimately, alleviating long term disabilities and related costs to our society. Sinc we address a generalized novel concept for imaging stem cell engraftment, results might not only impact patients with MASI but also patients with a variety of other (stem) cell transplants.

Public Health Relevance

The development of a non-invasive imaging technique for differentiation between viable and non-viable donor stem cells is crucial for monitoring of virtually any stem cell based therapy. A better understanding of the signal behavior of contrast agent labeled viable and apoptotic hMSC on MR images could help to investigate the mechanisms that control stem cell death and lead the way to a more effective use of hMSC-based therapies for arthritis. Results should be immediately helpful in preclinical assessments of hMSC-based therapies of arthritis and other joint pathologies, in the design of related clinical trials, and later, in the assessment of those stem cell based therapies in clinical practice.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Project (R01)
Project #
2R01AR054458-05
Application #
8294502
Study Section
Skeletal Biology Structure and Regeneration Study Section (SBSR)
Program Officer
Lester, Gayle E
Project Start
2006-12-01
Project End
2017-07-31
Budget Start
2012-08-01
Budget End
2013-07-31
Support Year
5
Fiscal Year
2012
Total Cost
$421,423
Indirect Cost
$155,069
Name
Stanford University
Department
Radiation-Diagnostic/Oncology
Type
Schools of Medicine
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305
Daldrup-Link, Heike E; Nejadnik, Hossein (2014) MR Imaging of Stem Cell Transplants in Arthritic Joints. J Stem Cell Res Ther 4:165
Khurana, Aman; Nejadnik, Hossein; Chapelin, Fanny et al. (2013) Ferumoxytol: a new, clinically applicable label for stem-cell tracking in arthritic joints with MRI. Nanomedicine (Lond) 8:1969-83
Khurana, Aman; Chapelin, Fanny; Beck, Graham et al. (2013) Iron administration before stem cell harvest enables MR imaging tracking after transplantation. Radiology 269:186-97
Nejadnik, Hossein; Daldrup-Link, Heike E (2012) Engineering stem cells for treatment of osteochondral defects. Skeletal Radiol 41:1-4
Castaneda, Rosalinda T; Khurana, Aman; Khan, Ramsha et al. (2011) Labeling stem cells with ferumoxytol, an FDA-approved iron oxide nanoparticle. J Vis Exp :e3482
Golovko, Daniel; Meier, Reinhard; Rummeny, Ernst et al. (2011) Optical imaging of rheumatoid arthritis. Int J Clin Rheumtol 6:67-75
Boddington, Sophie E; Sutton, Elizabeth J; Henning, Tobias D et al. (2011) Labeling human mesenchymal stem cells with fluorescent contrast agents: the biological impact. Mol Imaging Biol 13:3-9
Sutton, Elizabeth Jane; Henning, Tobias D; Boddington, Sophie et al. (2010) In vivo magnetic resonance imaging and optical imaging comparison of viable and nonviable mesenchymal stem cells with a bifunctional label. Mol Imaging 9:278-90
Nedopil, Alexander J; Mandrussow, Lydia G; Daldrup-Link, Heike E (2010) Implantation of ferumoxides labeled human mesenchymal stem cells in cartilage defects. J Vis Exp :
Meier, Reinhard; Krug, Christian; Golovko, Daniel et al. (2010) Indocyanine green-enhanced imaging of antigen-induced arthritis with an integrated optical imaging/radiography system. Arthritis Rheum 62:2322-7

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