Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA035381-13
Application #
2088943
Study Section
Bio-Organic and Natural Products Chemistry Study Section (BNP)
Project Start
1986-09-01
Project End
1997-02-28
Budget Start
1996-03-01
Budget End
1997-02-28
Support Year
13
Fiscal Year
1996
Total Cost
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
Schools of Pharmacy
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
Huang, Yong; Wendt-Pienkowski, Evelyn; Shen, Ben (2006) A dedicated phosphopantetheinyl transferase for the fredericamycin polyketide synthase from Streptomyces griseus. J Biol Chem 281:29660-8
Wendt-Pienkowski, Evelyn; Huang, Yong; Zhang, Jian et al. (2005) Cloning, sequencing, analysis, and heterologous expression of the fredericamycin biosynthetic gene cluster from Streptomyces griseus. J Am Chem Soc 127:16442-52
Wohlert, S E; Wendt-Pienkowski, E; Bao, W et al. (2001) Production of aromatic minimal polyketides by the daunorubicin polyketide synthase genes reveals the incompatibility of the heterologous DpsY and JadI cyclases. J Nat Prod 64:1077-80
Bao, W; Sheldon, P J; Wendt-Pienkowski, E et al. (1999) The Streptomyces peucetius dpsC gene determines the choice of starter unit in biosynthesis of the daunorubicin polyketide. J Bacteriol 181:4690-5
Bao, W; Sheldon, P J; Hutchinson, C R (1999) Purification and properties of the Streptomyces peucetius DpsC beta-ketoacyl:acyl carrier protein synthase III that specifies the propionate-starter unit for type II polyketide biosynthesis. Biochemistry 38:9752-7
Kendrew, S G; Katayama, K; Deutsch, E et al. (1999) DnrD cyclase involved in the biosynthesis of doxorubicin: purification and characterization of the recombinant enzyme. Biochemistry 38:4794-9
Bao, W; Wendt-Pienkowski, E; Hutchinson, C R (1998) Reconstitution of the iterative type II polyketide synthase for tetracenomycin F2 biosynthesis. Biochemistry 37:8132-8
Seow, K T; Meurer, G; Gerlitz, M et al. (1997) A study of iterative type II polyketide synthases, using bacterial genes cloned from soil DNA: a means to access and use genes from uncultured microorganisms. J Bacteriol 179:7360-8
Shen, B; Hutchinson, C R (1996) Deciphering the mechanism for the assembly of aromatic polyketides by a bacterial polyketide synthase. Proc Natl Acad Sci U S A 93:6600-4
Decker, H; Rohr, J; Motamedi, H et al. (1995) Identification of Streptomyces olivaceus Tu 2353 genes involved in the production of the polyketide elloramycin. Gene 166:121-6

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