Electron Paramagnetic Resonance (EPR) Oxygen Images (EPROI) provide quantitative localized pO2 distributions in animal tumors of syngeneic mice, rats, and rabbits. The oxygen broadening of narrow EPR spectral lines reports the pO2 with 1-3 torr resolution in each 1mm3 image voxel. This work has successfully shown 1) the first co-localized correlation between an oxygen image and a point oxygen measurement technique (Oxylite);2) independent, statistically significant sharpening of the 50% tumor control dose with the addition of the information provided by the fraction of EPROI voxels with pO2 less than 10 torr (HF10);3) significant spatial correlation of VEGF and EPROI based HF10;4) when treated to the dose necessary to control 50% (TCD50) of all FSa fibrosarcomas, control is only 28% in tumors with HF10>8% while it increases to 92% for those with HF10<8%. In the next funding cycle we propose to explore EPROI as the basis for IMRT dose painting in 2cm rat 13762 carcinomas, chosen for ease of IMRT via increased size. We propose the following specific aims for the next funding cycle: exploring the effectiveness of 1) Adding a fixed dose to the TCD50 targeting an IMRT boost to an EPROI based hypoxic region, compared with adding the same boost dose to a well oxygenated tumor region of equal volume 2) Adding dose to the hypoxic region and reduced dose to a well oxygenated region. This would be compared with delivery of uniform TCD50. 3) Adding a graded dose to regions of the tumor proportional to the HF10. This work will test the efficacy of the use of oxygen imaging in dose painting in small mammals. It will provide a path to enhancement of radiation therapy efficacy and reduction of its side effects.

Public Health Relevance

This work continues the investigation of a new technique to predict cure likelihood using radiation therapy for cancers and to improve the cure of cancers with radiation. The technique involves a non-invasive electron magnetic resonance image of a non-toxic compound, injected in an animal, which reports the amount of oxygen in each of the 1 mm3 volumes of the animal tumor. This eventually will guide the placement of extra radiation dose to enhance human tumor treatment with radiation.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA098575-08
Application #
8452054
Study Section
Radiation Therapeutics and Biology Study Section (RTB)
Program Officer
Bernhard, Eric J
Project Start
2003-06-01
Project End
2017-03-31
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
8
Fiscal Year
2013
Total Cost
$262,533
Indirect Cost
$94,243
Name
University of Chicago
Department
Radiation-Diagnostic/Oncology
Type
Schools of Medicine
DUNS #
005421136
City
Chicago
State
IL
Country
United States
Zip Code
60637
Epel, Boris; Maggio, Matt; Pelizzari, Charles et al. (2017) Electron Paramagnetic Resonance pO2 Image Tumor Oxygen-Guided Radiation Therapy Optimization. Adv Exp Med Biol 977:287-296
Rahimi, Robabeh; Halpern, Howard J; Takui, Takeji (2017) In Vivo EPR Resolution Enhancement Using Techniques Known from Quantum Computing Spin Technology. Adv Exp Med Biol 977:335-339
Kuzhelev, Andrey A; Tormyshev, Victor M; Rogozhnikova, Olga Yu et al. (2017) Triarylmethyl Radicals: EPR Study of 13C Hyperfine Coupling Constants. Z Phys Chem (N F) 231:777-794
Shi, Yilin; Quine, Richard W; Rinard, George A et al. (2017) Triarylmethyl Radical: EPR Signal to Noise at Frequencies between 250 MHz and 1.5 GHz and Dependence of Relaxation on Radical and Salt Concentration and on Frequency. Z Phys Chem (N F) 231:923-937
Boris, Epel; Sundramoorthy, Subramanian V; Halpern, Howard J (2017) 250 MHz passive Q-modulator for reflection resonators. Concepts Magn Reson Part B Magn Reson Eng 47B:
Epel, Boris; Sundramoorthy, Subramanian V; Krzykawska-Serda, Martyna et al. (2017) Imaging thiol redox status in murine tumors in vivo with rapid-scan electron paramagnetic resonance. J Magn Reson 276:31-36
Shi, Yilin; Quine, Richard W; Rinard, George A et al. (2017) Triarylmethyl Radical OX063d24 Oximetry: Electron Spin Relaxation at 250 MHz and RF Frequency Dependence of Relaxation and Signal-to-Noise. Adv Exp Med Biol 977:327-334
Rinard, George A; Quine, Richard W; Buchanan, Laura A et al. (2017) Resonators for In Vivo Imaging: Practical Experience. Appl Magn Reson 48:1227-1247
Sundramoorthy, Subramanian V; Epel, Boris; Halpern, Howard J (2017) A Pulse EPR 25 mT magnetometer with 10ppm resolution. Appl Magn Reson 48:805-811
Nakagawa, Kouichi; Epel, Boris (2017) Investigating the Distribution of Stable Paramagnetic Species in an Apple Seed Using X-Band EPR and EPR Imaging. J Oleo Sci 66:315-319

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