Although treatment for children with high-risk metastatic neuroblastoma has improved significantly in the past 20 years, only 45% of these patients become long-term, disease-free survivors, and bone marrow is a frequent site of resistant or recurrent disease. Improvement in survival necessitates effective new therapies for patients based upon biologically based risk stratification at diagnosis and highly sensitive monitoring of response to therapy that will identify patient subgroups with different outcomes who currently are clinically indistinguishable. High-risk neuroblastomas have few recurrent somatic mutations, which obviates molecular classification and therapies that rely on frequently altered oncogenic drivers. We have developed novel and clinically applicable TaqMan(R) Low Density Array (TLDA) gene expression assays for prognostication at diagnosis and for quantifying "tumor load" in bone marrow and blood for this group of patients. Overall goal: Validate the clinical utility of these new molecular diagnostics in collaboration with the Children's Oncology Group (COG) and then establish them as CAP/CLIA-accredited tests.
Specific Aims : Confirm performance characteristics and clinical utility 1) of a prediction signature (14-gene TLDA assay) for untreated primary MYCN non-amplified neuroblastomas and 2) of a detection signature (5-gene TLDA assay) for quantifying "tumor load" in bone marrow and blood. 3) Determine if these assays alone or combined improve prediction of outcome in the context of imaging and clinical variables. Research Design and Methods: The performance characteristics and clinical utility of the TLDA assays for risk stratification and monitoring of neuroblastoma response will be validated, and tests will be installed in CAP/CLIA-accredited laboratories including those at Children's Hospital Los Angeles [CHLA] and Nationwide Children's/COG Biopathology Center [BPC] so that they may be used in the future for clinical decision-making. Analytical performance characteristics of these tests will be confirmed in both CHLA and BPC laboratories, and clinical utility studies will be performed at CHLA. The 14-gene prediction signature predicted outcome in three independent clinical trials, and we are confident that additional studies using 300 archived and prospective samples from four COG studies will replicate existing data. Initial studies of the 5-gene detection signature (CHGA, DCX, DDC, PHOX2B, and TH), which can identify one tumor cell among 106 normal bone marrow or blood cells, showed that tumor load in bone marrow and PBSC during induction, after myeloablative consolidation, and after post-consolidation therapy correlates with outcome. These results will be validated with 2,250 archived and prospective bone marrow, blood, and PBSC specimens from four COG studies. Comprehensive analyses aimed at optimizing stratification and monitoring for clinical decision making will include these molecular diagnostic tests, 131I-meta-iodobenzyguanidine (MIBG) imaging, and clinical variables. Summary: We anticipate that our moelcular diagnostic assays will improve prediction of outcome and development of more effective therapy for patients with high-risk metastatic neuroblastoma.

Public Health Relevance

Although treatment has steadily improved in the past 20 years for children with high-risk metastatic neuroblastoma (stage 4), only 45% of these patients survive long-term, and bone marrow is a frequent site of resistant or recurrent disease. We have developed clinically applicable gene expression assays for risk stratification at diagnosis and for monitoring response to treatment in bone marrow and blood during and after therapy. We anticipate that these assays will become integral biomarker tools for developing more effective therapy for patients with high-risk metastatic neuroblastoma.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
1R01CA182633-01
Application #
8616217
Study Section
Clinical Oncology Study Section (CONC)
Program Officer
Thurin, Magdalena
Project Start
2014-03-04
Project End
2017-02-28
Budget Start
2014-03-04
Budget End
2015-02-28
Support Year
1
Fiscal Year
2014
Total Cost
$704,800
Indirect Cost
$257,715
Name
Children's Hospital of Los Angeles
Department
Type
DUNS #
052277936
City
Los Angeles
State
CA
Country
United States
Zip Code
90027