Drug abuse and dependence define behavioral states involving increased allocation of behavior towards drug seeking and taking at the expense of more appropriate behavioral patterns. As such, addiction can be viewed as increased control of behavior by the desired drug (due to its unconditioned, rewarding properties). It is also clear that drug-associated (conditioned) stimuli acquire heightened abilities to control behavior. These phenomena have been linked with dopamine (DA) function within the ventral striatum and amygdala and have been described specifically in terms of incentive motivational processes. Our data show that persistent drug-induced enhancement in incentive motivation are associated with, and can be mimicked by, increases in limbic-striatal DA/cAMP/PKA/CREB activity, suggesting that adaptations in DA-regulated intracellular signaling molecules may underlie these behavioral changes. The current proposal will determine how PKA/CREB signaling within the amygdala contributes to associative aspects of incentive motivational processes by using Pavlovian-to-lnstrumental Transfer (PIT) and responding for Conditioned Reinforcement (CR) paradigms. This project will focus on the hypothesis that repeated cocaine exposure will result in persistent enhancements in incentive motivational processes due to alterations in the PKA/CREB signaling pathway in the amygdala. Specifically, we will investigate the following hypotheses. (1) Repeated cocaine exposure enhances incentive motivation and results in alterations of PKA/CREB FosB function in the amygdala. Behavioral and molecular alterations will be examined in rats previously-exposed to cocaine either responding in the presence of (PIT) or for (CR) food-paired conditioned stimuli. (2) Activation of PKA/CREB signaling in drug naive animals will augment incentive motivation. This will be examined using infusions of direct activators/inhibitors of PKA and viral vectors that over-express CREB or mutant CREB into the basolateral or central nucleus of the amygdala. (3) Inhibition of PKA/CREB signaling will block augmented incentive motivation in cocaine-treated animals. Correlations between behavior and CREB activity will also be examined in CRE-LacZ transgenic mice. Together these studies will determine whether cocaine-induced activation of the PKA/CREB signaling pathway in the amygdala results in augmented incentive motivation and whether these effects can be mimicked or blocked by manipulations that stimulate or inhibit PKA/CREB signaling, respectively. These data may be critical for understanding the neurobiology of motivational processes and their putative impact on drug-seeking behavior. Persistent alterations in PKA/CREB signaling in limbic-striatal regions produced by cocaine are hypothesized to contribute to compulsive reward seeking in addiction.

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Research Project (R01)
Project #
5R01DA015222-04
Application #
7092248
Study Section
Special Emphasis Panel (ZRG1-SSS-C (01))
Program Officer
Volman, Susan
Project Start
2003-09-30
Project End
2008-06-30
Budget Start
2006-07-01
Budget End
2007-06-30
Support Year
4
Fiscal Year
2006
Total Cost
$261,556
Indirect Cost
Name
Yale University
Department
Psychiatry
Type
Schools of Medicine
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520
Dunbar, Amber B; Taylor, Jane R (2017) Garcinol Blocks the Reconsolidation of Multiple Cocaine-Paired Cues after a Single Cocaine-Reactivation Session. Neuropsychopharmacology 42:1884-1892
Monsey, Melissa S; Sanchez, Hayde; Taylor, Jane R (2017) The Naturally Occurring Compound Garcinia Indica Selectively Impairs the Reconsolidation of a Cocaine-Associated Memory. Neuropsychopharmacology 42:587-597
Dunbar, Amber B; Taylor, Jane R (2017) Reconsolidation and psychopathology: Moving towards reconsolidation-based treatments. Neurobiol Learn Mem 142:162-171
Dunbar, Amber B; Taylor, Jane R (2016) Inhibition of protein synthesis but not ?-adrenergic receptors blocks reconsolidation of a cocaine-associated cue memory. Learn Mem 23:391-8
Rich, Matthew T; Abbott, Thomas B; Chung, Lisa et al. (2016) Phosphoproteomic Analysis Reveals a Novel Mechanism of CaMKII? Regulation Inversely Induced by Cocaine Memory Extinction versus Reconsolidation. J Neurosci 36:7613-27
Honsberger, Michael J; Taylor, Jane R; Corlett, Philip R (2015) Memories reactivated under ketamine are subsequently stronger: A potential pre-clinical behavioral model of psychosis. Schizophr Res 164:227-33
Harb, Roa; Taylor, Jane R; Taulor, Jane R (2015) The fragrant power of collective fear. PLoS One 10:e0123908
Taylor, Jane R; Torregrossa, Mary M (2015) Pharmacological disruption of maladaptive memory. Handb Exp Pharmacol 228:381-415
Quick, Stacey L; Olausson, Peter; Addy, Nii A et al. (2014) Repeated nicotine exposure during adolescence alters reward-related learning in male and female rats. Behav Brain Res 261:171-6
Wan, Xun; Torregrossa, Mary M; Sanchez, Hayde et al. (2014) Activation of exchange protein activated by cAMP in the rat basolateral amygdala impairs reconsolidation of a memory associated with self-administered cocaine. PLoS One 9:e107359

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