Tobacco abuse among adolescents is a major health and economic concern, yet there is a critical knowledge gap regarding the mechanisms that mediate adolescent smoking behavior. Our preliminary data demonstrate that adolescent male rats display enhanced nicotine reward and reduced nicotine withdrawal relative to adults. This proposal extends our previous work by examining the neural mechanisms that mediate developmental sensitivity and sex differences in the behavioral .effects of nicotine reward and withdrawal. In order to study the long-term consequences produced by adolescent nicotine exposure, our proposal also includes adult rats pre-exposed to nicotine during adolescence. Nicotine reward is mediated in part by enhanced dopamine (DA) transmission in the nucleus accumbens (NAcc). In contrast, nicotine withdrawal produces a decrease in NAcc DA levels. Changes in NAcc DA transmission are mediated by excitatory and inhibitory systems that regulate DA projections that originate in the ventral tegmental area (VTA). During adolescence, excitatory systems that regulate DA are overdeveloped, whereas inhibitory systems are underdeveloped. We hypothesize that adolescents will display enhanced nicotine reward and reduced nicotine withdrawal relative to adults. These developmental differences are likely mediated via enhanced excitation and reduced inhibition of VTA DA cell bodies. Overall we expect that these differences will be magnified in female adolescents and nicotine pre-exposed rats. This is based on clinical studies demonstrating that teenage females and adults pre-exposed to nicotine during adolescence display enhanced vulnerability to long-term tobacco abuse. Behavioral studies will compare nicotine reward using place preference procedures and nicotine withdrawal using place aversion procedures. Neurochemical studies will employ in vivo microdialysis procedures to compare NAcc DA and concomitant measures of excitatory and inhibitory systems in the VTA following nicotine administration and during nicotine withdrawal. This research is significant because our findings may have significant implications towards guiding public policies regarding tobacco regulation and warnings directed at teenagers and females. Further, a better understanding of the mechanisms mediating adolescent tobacco abuse will lead to more effective treatments for young smokers who are most at risk of developing diseases caused by long-term smoking.

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Research Project (R01)
Project #
5R01DA021274-06
Application #
8249850
Study Section
Neurobiology of Motivated Behavior Study Section (NMB)
Program Officer
Lynch, Minda
Project Start
2007-08-01
Project End
2013-12-31
Budget Start
2012-04-01
Budget End
2013-03-31
Support Year
6
Fiscal Year
2012
Total Cost
$270,114
Indirect Cost
$76,249
Name
University of Texas El Paso
Department
Psychology
Type
Schools of Arts and Sciences
DUNS #
132051285
City
El Paso
State
TX
Country
United States
Zip Code
79968
Carcoba, Luis M; Orfila, James E; Natividad, Luis A et al. (2014) Cholinergic transmission during nicotine withdrawal is influenced by age and pre-exposure to nicotine: implications for teenage smoking. Dev Neurosci 36:347-55
O'Dell, Laura E; Torres, Oscar V (2014) A mechanistic hypothesis of the factors that enhance vulnerability to nicotine use in females. Neuropharmacology 76 Pt B:566-80
Torres, Oscar V; Walker, Ellen M; Beas, Blanca S et al. (2014) Female rats display enhanced rewarding effects of ethanol that are hormone dependent. Alcohol Clin Exp Res 38:108-15
O'Dell, Laura E; Natividad, Luis A; Pipkin, Joseph A et al. (2014) Enhanced nicotine self-administration and suppressed dopaminergic systems in a rat model of diabetes. Addict Biol 19:1006-19
Torres, Oscar V; Gentil, Luciana G; Natividad, Luis A et al. (2013) Behavioral, Biochemical, and Molecular Indices of Stress are Enhanced in Female Versus Male Rats Experiencing Nicotine Withdrawal. Front Psychiatry 4:38
Natividad, Luis A; Torres, Oscar V; Friedman, Theodore C et al. (2013) Adolescence is a period of development characterized by short- and long-term vulnerability to the rewarding effects of nicotine and reduced sensitivity to the anorectic effects of this drug. Behav Brain Res 257:275-85
Vuong, Cassidy; Van Uum, Stan H M; O'Dell, Laura E et al. (2010) The effects of opioids and opioid analogs on animal and human endocrine systems. Endocr Rev 31:98-132
Natividad, Luis A; Tejeda, Hugo A; Torres, Oscar V et al. (2010) Nicotine withdrawal produces a decrease in extracellular levels of dopamine in the nucleus accumbens that is lower in adolescent versus adult male rats. Synapse 64:136-45
O'Dell, Laura E (2009) A psychobiological framework of the substrates that mediate nicotine use during adolescence. Neuropharmacology 56 Suppl 1:263-78
Torres, Oscar V; Natividad, Luis A; Tejeda, Hugo A et al. (2009) Female rats display dose-dependent differences to the rewarding and aversive effects of nicotine in an age-, hormone-, and sex-dependent manner. Psychopharmacology (Berl) 206:303-12

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