Histologic study at the level of light and electron microscopy of normal and pathologic human inner ear and spiral ganglion is proposed.
The specific aims i nclude: 1. Completion of a serial section study of a case of Meniere's disease, including statistical analysis of the number of afferent endings at the base of inner and outer hair cells, the neuronal density at the level of the osseous spiral lamina and at the level of spiral ganglion. 2. Initiating a qualitative and qualitative analysis of the human spiral ganglion by serial section analysis and 3D reconstruction. After normative data is obtained, this analysis will be extended to pathological cases which have been already obtained and to specimens anticipated. 3. Continued study of human temporal bone material already obtained in the pervious grant period. The acquisition of human temporal bones has exceeded our capacity to study them by electron microscopy and there are several cases with audiometric documentation which have yet to be studied. 4. Continued accrual of temporal bones with audiometric documentation and of sufficiently good fixation for electron microscopy. The exact nature of study will obviously depend in large measure on pathology obtained in these cases. Temporal bones are obtained through the Pathology Departments of the affiliated hospitals and through the National Temporal Bone Bank. Specimens are fixed in situ and removed in the Pathology Department and prepared for light and electron microscopy. Analysis of material obtained would be largely by serial section reconstruction and 3D reconstruction as needed. There is a growing body of data suggesting that the normal ultrastructural anatomy of the human inner ear, both at the level of the organ of Corti and at that of the spiral ganglion, is significantly different from that of the usual mammalian models. Further documentation of these differences as well as pathologic changes seen in the human will be of use in interpreting animal models of hearing loss, understanding of human diseases of the inner ear, and providing a rational foundation for treatment of human hearing loss.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Project (R01)
Project #
5R01DC000152-16
Application #
2124860
Study Section
Hearing Research Study Section (HAR)
Project Start
1979-12-01
Project End
1996-01-31
Budget Start
1995-02-01
Budget End
1996-01-31
Support Year
16
Fiscal Year
1995
Total Cost
Indirect Cost
Name
Massachusetts Eye and Ear Infirmary
Department
Type
DUNS #
073825945
City
Boston
State
MA
Country
United States
Zip Code
02114
Trakimas, Danielle R; Kozin, Elliott D; Ghanad, Iman et al. (2018) Human Otopathologic Findings in Cases of Folded Cochlear Implant Electrodes. Otol Neurotol 39:970-978
Kamakura, Takefumi; O'Malley, Jennifer T; Nadol Jr, Joseph B (2018) Preservation of Cells of the Organ of Corti and Innervating Dendritic Processes Following Cochlear Implantation in the Human: An Immunohistochemical Study. Otol Neurotol 39:284-293
O'Malley, Jennifer T; Burgess, Barbara J; Galler, Donald et al. (2017) Foreign Body Response to Silicone in Cochlear Implant Electrodes in the Human. Otol Neurotol 38:970-977
Kamakura, Takefumi; Lee, Daniel J; Herrmann, Barbara S et al. (2017) Histopathology of the Human Inner Ear in the Cogan Syndrome with Cochlear Implantation. Audiol Neurootol 22:116-123
Ishai, Reuven; Herrmann, Barbara S; Nadol Jr, Joseph B et al. (2017) The pattern and degree of capsular fibrous sheaths surrounding cochlear electrode arrays. Hear Res 348:44-53
Kamakura, Takefumi; Nadol Jr, Joseph B (2016) Correlation between word recognition score and intracochlear new bone and fibrous tissue after cochlear implantation in the human. Hear Res 339:132-41
Kamakura, Takefumi; Nadol Jr, Joseph B (2016) Cochlear Histopathology as Observed in Two Patients With a Cochlear Implant Electrode With Positioner. Otol Neurotol 37:642-6
Burgess, Barbara J; O'Malley, Jennifer T; Kamakura, Takefumi et al. (2016) Histopathology of the Human Inner Ear in the p.L114P COCH Mutation (DFNA9). Audiol Neurootol 21:88-97
Quesnel, Alicia M; Nakajima, Hideko Heidi; Rosowski, John J et al. (2016) Delayed loss of hearing after hearing preservation cochlear implantation: Human temporal bone pathology and implications for etiology. Hear Res 333:225-234
Nadol Jr, Joseph B; Handzel, Ophir; Amr, Sami (2015) Histopathology of the Human Inner Ear in a Patient With Sensorineural Hearing Loss Caused by a Variant in DFNA5. Otol Neurotol 36:1616-21

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