Hearing begins with the detection of sound by hair cells in the cochlea of the inner ear. Spiral ganglion neurons provide the sole conduit for auditory information from hair cells to the central nervous system. Loss of auditory neurons occurs in response to injury, tumors, or hair cell degeneration, which are all common causes of human congenital and age-related deafness. The most effective treatment for deafness is the cochlear implant, which works by directly stimulating spiral ganglion neurons, emphasizing the need to maintain properly wired connections between the ear and the brain. Understanding how auditory neurons are patterned and wired during development will provide an important foundation for the design of therapies to protect inner ear neurons from degeneration and for the development of stem-cell based methods for neuronal replacement. A central question in auditory neuroscience is how spiral ganglion neurons acquire properties that are specific for the perception of sound. Spiral ganglion neurons originate together with vestibular ganglion neurons within a common neurogenic region of the otic vesicle. Precisely wired auditory circuits form through a series of events, including the extension of processes towards hair cells, bifurcation of projections in the cochlear nucleus, and the formation of specialized synapses with target neurons in the brainstem. Many of these events are highlighted by comparison with vestibular ganglion neurons, which underlie the perception of balance and therefore make a distinct series of wiring decisions within the same local environment. The transcription factor GATA3 is produced in auditory but not vestibular neurons. Based on its activity as a master regulator in other developing systems, GATA3 is hypothesized to coordinate auditory-specific programs of development in spiral ganglion neurons. There are three goals: 1) to compare gene expression profiles in highly purified spiral and vestibular ganglion neurons in order to define the auditory-specific programs of circuit assembly underlying specific wiring events 2) to understand how GATA3 exerts distinct effects on early and late wiring events by generating and analyzing conditional knock-out mice;and 3) to identify auditory-specific genes that act downstream of GATA3 to regulate multiple stages of circuit formation. Results from these experiments will provide key insights into the unique cellular and molecular properties of auditory neurons, and may shed light on the etiology of deafness associated with hypoparathyroidism, sensorineural deafness, and renal anomalies (HDR), which is caused by mutations in GATA3.

Public Health Relevance

Sounds are collected by the ear, but we only become aware of sounds because of the activity of complex networks of neurons that connect the ear to the brain. Auditory neurons can die due to traumatic injury or because other parts of the ear do not function properly. An effective treatment for deafness is the cochlear implant, which replaces the cochlea by directly stimulating auditory neurons. Understanding how specific sets of neurons become uniquely suited for the perception of sound is a crucial step towards improved cochlear implant technology, and could help identify new ways to keep neurons alive or to replace damaged neurons with new neurons that can re-establish precise connections between the ear and the brain.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Project (R01)
Project #
5R01DC009223-05
Application #
8446409
Study Section
Auditory System Study Section (AUD)
Program Officer
Freeman, Nancy
Project Start
2009-05-15
Project End
2014-03-31
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
5
Fiscal Year
2013
Total Cost
$327,916
Indirect Cost
$134,455
Name
Harvard University
Department
Biology
Type
Schools of Medicine
DUNS #
047006379
City
Boston
State
MA
Country
United States
Zip Code
02115
Yu, Wei-Ming; Goodrich, Lisa V (2014) Morphological and physiological development of auditory synapses. Hear Res 311:3-16
Appler, Jessica M; Lu, Cindy C; Druckenbrod, Noah R et al. (2013) Gata3 is a critical regulator of cochlear wiring. J Neurosci 33:3679-91
Lu, Cindy C; Appler, Jessica M; Houseman, E Andres et al. (2011) Developmental profiling of spiral ganglion neurons reveals insights into auditory circuit assembly. J Neurosci 31:10903-18
Appler, Jessica M; Goodrich, Lisa V (2011) Connecting the ear to the brain: Molecular mechanisms of auditory circuit assembly. Prog Neurobiol 93:488-508