Diabetes increases the risk of severe infections, including periodontitis. Maintenance of homeostasis and tissue health of the periodontium is complicated by overgrowth of specific pathogens. The innate immune response prevents invasion and limits the complexity of the infection. When disease occurs as a complication of systemic disease as in diabetes, tissue damage results from the host response (phagocyte mediated tissue injury), which provides a microenvironment that favors bacterial growth and the chronicity of the lesion. Dysregulated inflammatory/immune reactions have been implicated in the pathogenesis of the complications of diabetes, particularly periodontitis and cardiovascular diseases. Tissues exposed to the external environment and significant bacteria load, such as the periodontium, are at increased risk. The return of inflamed tissues to health and homeostasis is mediated by endogenous agonists that promote resolution of inflammation. The cellular and molecular mechanisms responsible for the resolution of periodontal inflammation are beginning to be mapped. These same proresolution agonists enhance host cell mediated antibacterial activity. Precursor fatty acid substrates from cells (arachidonic acid) and dietary sources (omega-3 fatty acids) yield lipid mediators (lipoxins and resolvins, respectively) that counter-regulate pro-inflammatory signals. Systematic temporal study of periodontal infection and resolution in tissues of people with diabetes is of paramount importance in the treatment of bacterially initiated periodontal disease. The goal of this competing renewal R01 proposal is to determine the impact of infection in resolution circuits of inflammation in people with diabetes. Our novel hypothesis is that restoration of resolution pathways by exogenous administration of resolving molecules will resolve periodontal inflammation and restore homeostasis modifying the composition of the microflora. In this proposal, we will investigate the mechanism of action of phagocyte interactions in the periodontium invoking innate antimicrobial activity and enhanced clearance of pathogens in people with diabetes.

Public Health Relevance

Diabetes increases the risk of severe infections, including periodontitis. When periodontal disease occurs as a complication of systemic disease as in diabetes, tissue damage results from the host response and the return of inflamed tissues to health is mediated by molecules that promote resolution of inflammation. The goal of this competing renewal R01 proposal is to determine the impact of infection in resolution circuits of inflammation in people with diabetes.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE015566-10
Application #
8495759
Study Section
Special Emphasis Panel (ZRG1-MOSS-B (03))
Program Officer
Lunsford, Dwayne
Project Start
2003-12-01
Project End
2015-06-30
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
10
Fiscal Year
2013
Total Cost
$465,113
Indirect Cost
$198,414
Name
Forsyth Institute
Department
Type
DUNS #
062190616
City
Cambridge
State
MA
Country
United States
Zip Code
02142
Steffens, J P; Herrera, B S; Coimbra, L S et al. (2014) Testosterone regulates bone response to inflammation. Horm Metab Res 46:193-200
Gaffen, S L; Herzberg, M C; Taubman, M A et al. (2014) Recent advances in host defense mechanisms/therapies against oral infectious diseases and consequences for systemic disease. Adv Dent Res 26:30-7
Batista Jr, Eraldo L; Kantarci, Alpdogan I; Hasturk, Hatice et al. (2014) Alternative splicing generates a diacylglycerol kinase ? transcript that acts as a dominant-negative modulator of superoxide production in localized aggressive periodontitis. J Periodontol 85:934-43
Martin, Camilia R; Zaman, Munir M; Gilkey, Calvin et al. (2014) Resolvin D1 and lipoxin A4 improve alveolarization and normalize septal wall thickness in a neonatal murine model of hyperoxia-induced lung injury. PLoS One 9:e98773
Janket, Sok-Ja; Baird, Alison E; Jones, Judith A et al. (2014) Number of teeth, C-reactive protein, fibrinogen and cardiovascular mortality: a 15-year follow-up study in a Finnish cohort. J Clin Periodontol 41:131-40
Zenobia, Camille; Hasturk, Hatice; Nguyen, Daniel et al. (2014) Porphyromonas gingivalis lipid A phosphatase activity is critical for colonization and increasing the commensal load in the rabbit ligature model. Infect Immun 82:650-9
Zhu, Min; Belkina, Anna C; DeFuria, Jason et al. (2014) B cells promote obesity-associated periodontitis and oral pathogen-associated inflammation. J Leukoc Biol 96:349-57
Freire, Marcelo O; Van Dyke, Thomas E (2013) Natural resolution of inflammation. Periodontol 2000 63:149-64
Bartold, P Mark; Van Dyke, Thomas E (2013) Periodontitis: a host-mediated disruption of microbial homeostasis. Unlearning learned concepts. Periodontol 2000 62:203-17
Hasturk, Hatice; Kantarci, Alpdogan; Van Dyke, Thomas E (2012) Paradigm shift in the pharmacological management of periodontal diseases. Front Oral Biol 15:160-76

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