The overall goal of this project is to elucidate interacting endogenous anti-inflammatory pathways that can be harnessed to suppress an over-exuberant host response to periodontal bacteria and other pathogens. Bacteria are initially recognized by Toll-like receptors (TLRs) and other pathogen recognition receptors expressed on innate cells. TLR interaction with microbe-specific molecular patterns induces the innate production of pro- inflammatory cytokines, which direct the intensity and direction of the immune response, leads to recruitment of leukocytes to the site of infection, and the production of an arsenal of anti-bacterial molecules, many of which have the potential to cause the collateral tissue damage that defines destructive inflammatory diseases. There are at least three endogenous anti-inflammatory pathways. The first life cycle of DE017680 focused on the in vitro elucidation of the PI3K/ AKT anti-inflammatory pathway, the key role of GSK3b in this signaling cascade, and the mechanisms by which this pathway regulates the expression of IFNb, IL-1Ra, IL-10 in innate cells in addition to controlling T cell development and proliferation. In addition o the PI3K/ AKT cascade, this renewal will also consider the a7 nicotinic acetylcholine receptor-dependent cholinergic and the Wnt3a anti- inflammatory pathways. Crosstalk between these three pathways will be elucidated, with particular attention paid to the pivotal role of the centra inflammatory mediator, GSK3b, in pathway convergence. While each pathway is efficacious in its own right, therapeutic interventions that maximize the anti-inflammatory potential of convergent signaling events are likely to be particularly potent. To this end, anti-inflammatory signaling events will be exploited using murine models of inflammation (sub-cutaneous chamber) and periodontitis (alveolar bone loss). Therefore, this project will lay the groundwork for the development of novel approaches to the treatment of periodontitis and other inflammatory diseases and identify therapeutic targets that should be readily translatable.
It is the host response to dental plaque that leads to the development of periodontitis, one of the most common infectious diseases in humans that consumes >$14 billion dollars in health costs in the U.S. per annum. We will identify overlapping events between several endogenous mechanisms of inflammatory suppression (the PI3K/AKT/GSK3b;cholinergic;and Wnt3a anti-inflammatory pathways);elucidate specific mechanisms that suppress pro-inflammatory cytokine production in response to bacterial infection;determine anti-inflammatory events that control the release of tissue degrading matrix metalloproteinases;and establish if we can exploit these anti-inflammatory pathways to reduce inflammation and periodontal disease progression in murine models. These data will identify novel potential therapeutic approaches to the control of destructive inflammation.
|Wang, Huizhi; Zhou, Huaxin; Duan, Xiaoxian et al. (2014) Porphyromonas gingivalis-induced reactive oxygen species activate JAK2 and regulate production of inflammatory cytokines through c-Jun. Infect Immun 82:4118-26|
|Wang, Huizhi; Kumar, Akhilesh; Lamont, Richard J et al. (2014) GSK3? and the control of infectious bacterial diseases. Trends Microbiol 22:208-17|
|Bondy-Carey, J L; Galicia, J; Bagaitkar, J et al. (2013) Neutrophils alter epithelial response to Porphyromonas gingivalis in a gingival crevice model. Mol Oral Microbiol 28:102-13|
|Wang, Huizhi; Brown, Jonathan; Gu, Zhen et al. (2011) Convergence of the mammalian target of rapamycin complex 1- and glycogen synthase kinase 3-?-signaling pathways regulates the innate inflammatory response. J Immunol 186:5217-26|
|Wang, Huizhi; Brown, Jonathan; Garcia, Carlos A et al. (2011) The role of glycogen synthase kinase 3 in regulating IFN-?-mediated IL-10 production. J Immunol 186:675-84|
|Brown, J; Wang, H; Hajishengallis, G N et al. (2011) TLR-signaling networks: an integration of adaptor molecules, kinases, and cross-talk. J Dent Res 90:417-27|
|Brown, Jonathan; Wang, Huizhi; Suttles, Jill et al. (2011) Mammalian target of rapamycin complex 2 (mTORC2) negatively regulates Toll-like receptor 4-mediated inflammatory response via FoxO1. J Biol Chem 286:44295-305|
|Wang, Huizhi; Brown, Jonathan; Martin, Michael (2011) Glycogen synthase kinase 3: a point of convergence for the host inflammatory response. Cytokine 53:130-40|
|Rehani, Kunal; Wang, Huizhi; Garcia, Carlos A et al. (2009) Toll-like receptor-mediated production of IL-1Ra is negatively regulated by GSK3 via the MAPK ERK1/2. J Immunol 182:547-53|
|Garcia, Carlos A; Wang, Huizhi; Benakanakere, Manjunatha R et al. (2009) c-jun controls the ability of IL-12 to induce IL-10 production from human memory CD4+ T cells. J Immunol 183:4475-82|
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