Moisture is the nemesis of strong polymer adhesion to metals and minerals. Most engineered adhesive polymers require extensive prior surface cleaning, drying, and sometimes even chemical modification for effective adhesion to polar surfaces. Such surface preparation is difficult in vivo since biomineralized tissues and implant material surfaces are necessarily hydrated within the body. Various marine organisms have evolved highly effective adhesive strategies for wet surfaces. The broad goal of this proposal is to obtain mechanistic information about marine adhesion in order to translate it into effective applications for restoration and repair of hard tissues. While the discovery of 3,4-dihydroxyphenylalanine (Dopa)-protein involvement in adhesion has already inspired several new biomedical materials, Dopa is not the only bioinspired theme.
The specific aims here are to determine using mass spectrometry whether and to what extent phosphoserine and 4-hydroxyarginine are linked to mussel adhesion on different surfaces, characterize the specific protein-protein interactions during adhesive cross-linking, and to explore how factors such as mass, primary sequence, and side- chain functionalization influence the coating or bridging behavior of mfp-1 on surfaces such as titanium and hydroxyapatite using the surface forces apparatus. Bio-inspired adhesives and sealants are much needed in dentistry and orthopedics not just to improve the strength and durability of bonding to hard tissues, but also to emancipate the present technology, particularly in dentistry, from a reliance on highly reactive and toxic organic formulations.

Public Health Relevance

In dental and biomedical restorations, water is the nemesis of true adhesion between solid surfaces and polymers. The strong underwater adhesion of marine organisms such as mussels is based on an adaptive set of molecular and biophysical properties that will be systematically translated into medically relevant strategies.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE018468-05
Application #
8286067
Study Section
Biomaterials and Biointerfaces Study Section (BMBI)
Program Officer
Drummond, James
Project Start
2008-08-04
Project End
2013-08-31
Budget Start
2012-07-01
Budget End
2013-08-31
Support Year
5
Fiscal Year
2012
Total Cost
$453,463
Indirect Cost
$143,955
Name
University of California Santa Barbara
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
094878394
City
Santa Barbara
State
CA
Country
United States
Zip Code
93106
Kan, Yajing; Danner, Eric W; Israelachvili, Jacob N et al. (2014) Boronate complex formation with Dopa containing mussel adhesive protein retards ph-induced oxidation and enables adhesion to mica. PLoS One 9:e108869
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Akdogan, Yasar; Wei, Wei; Huang, Kuo-Ying et al. (2014) Intrinsic surface-drying properties of bioadhesive proteins. Angew Chem Int Ed Engl 53:11253-6
Wei, Wei; Tan, Yerpeng; Martinez Rodriguez, Nadine R et al. (2014) A mussel-derived one component adhesive coacervate. Acta Biomater 10:1663-70
Wasko, S Scott; Tay, Gavin Z; Schwaighofer, Andreas et al. (2014) Structural proteins from whelk egg capsule with long range elasticity associated with a solid-state phase transition. Biomacromolecules 15:30-42
Tan, Yerpeng; Yildiz, Umit Hakan; Wei, Wei et al. (2013) Layer-by-layer polyelectrolyte deposition: a mechanism for forming biocomposite materials. Biomacromolecules 14:1715-26
Ortony, Julia H; Hwang, Dong Soo; Franck, John M et al. (2013) Asymmetric collapse in biomimetic complex coacervates revealed by local polymer and water dynamics. Biomacromolecules 14:1395-402
Nicklisch, Sascha C T; Das, Saurabh; Martinez Rodriguez, Nadine R et al. (2013) Antioxidant efficacy and adhesion rescue by a recombinant mussel foot protein-6. Biotechnol Prog 29:1587-93
Yu, Jing; Wei, Wei; Menyo, Matthew S et al. (2013) Adhesion of mussel foot protein-3 to TiO2 surfaces: the effect of pH. Biomacromolecules 14:1072-7
Lee, Dong Woog; Lim, Chanoong; Israelachvili, Jacob N et al. (2013) Strong adhesion and cohesion of chitosan in aqueous solutions. Langmuir 29:14222-9

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