Most bacterial pathogenesis studies have focused on mono-culture infections;however, it is clear that many bacterial infections are not simply the result of colonization with a single species, but rather ensue from the action of polymicrobial communities. Microbes within polymicrobial infections often display synergistic interactions that result in enhanced colonization and persistence in the infection site. Such interactions have been particularly noted in infections of the oral cavity, although the molecular processes controlling these synergistic interactions are generally not known. Detailed mechanistic studies elucidating the polymicrobial interactions necessary for enhanced persistence in vivo is critical for a comprehensive understanding of synergy, and a necessary first step towards developing therapeutics to treat polymicrobial infections. The overall goal of this research plan is to examine, from a mechanistic standpoint, how interactions between oral bacteria impact community development and in vivo persistence. To accomplish this goal, high-throughput genomics techniques will be employed to identify microbial virulence genes that are uniquely expressed during co-infection and/or required for polymicrobial synergy.
The survival of pathogens in the human body has been rigorously studied for well over a century. Most bacterial pathogenesis studies have focused on mono-culture infections;however, it is clear that many bacterial infections are not simply the result of colonization with a single species, but are instead a result of colonization with several Microbes within polymicrobial infections often display synergistic interactions that result in enhanced colonization and persistence in the infection site. The goal of this research proposal is to utilize high throughput genomics techniques to elucidate the molecular basis of polymicrobial synergy in two model oral polymicrobial communities, with the ultimate goal of devising new therapeutic strategies for treating such infections.
|Kuboniwa, Masae; Houser, John R; Hendrickson, Erik L et al. (2017) Metabolic crosstalk regulates Porphyromonas gingivalis colonization and virulence during oral polymicrobial infection. Nat Microbiol 2:1493-1499|
|Ibberson, Carolyn B; Stacy, Apollo; Fleming, Derek et al. (2017) Co-infecting microorganisms dramatically alter pathogen gene essentiality during polymicrobial infection. Nat Microbiol 2:17079|
|Miller, Daniel P; Hutcherson, Justin A; Wang, Yan et al. (2017) Genes Contributing to Porphyromonas gingivalis Fitness in Abscess and Epithelial Cell Colonization Environments. Front Cell Infect Microbiol 7:378|
|Ho, Meng-Hsuan; Lamont, Richard J; Xie, Hua (2017) A novel peptidic inhibitor derived from Streptococcus cristatus ArcA attenuates virulence potential of Porphyromonas gingivalis. Sci Rep 7:16217|
|Hendrickson, Erik L; Beck, David A C; Miller, Daniel P et al. (2017) Insights into Dynamic Polymicrobial Synergy Revealed by Time-Coursed RNA-Seq. Front Microbiol 8:261|
|Ho, Meng-Hsuan; Lamont, Richard J; Xie, Hua (2017) Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis. Sci Rep 7:1413|
|Narayanan, Ajay M; Ramsey, Matthew M; Stacy, Apollo et al. (2017) Defining Genetic Fitness Determinants and Creating Genomic Resources for an Oral Pathogen. Appl Environ Microbiol 83:|
|Michie, Kelly L; Cornforth, Daniel M; Whiteley, Marvin (2016) Bacterial tweets and podcasts #signaling#eavesdropping#microbialfightclub. Mol Biochem Parasitol 208:41-8|
|Hajishengallis, George; Lamont, Richard J (2016) Dancing with the Stars: How Choreographed Bacterial Interactions Dictate Nososymbiocity and Give Rise to Keystone Pathogens, Accessory Pathogens, and Pathobionts. Trends Microbiol 24:477-89|
|Hutcherson, J A; Gogeneni, H; Yoder-Himes, D et al. (2016) Comparison of inherently essential genes of Porphyromonas gingivalis identified in two transposon-sequencing libraries. Mol Oral Microbiol 31:354-64|
Showing the most recent 10 out of 24 publications