High risk human papilloma virus related head and neck squamous cell carcinoma (HPVHNSCC) incidence is rapidly increasing, and will soon surpass the incidence of cervical carcinoma in the US. There are no effective ways to detect preclinical or early HPVHNSCC, screen populations at increased risk for HPVHNSCC development, or even assay for microscopic persistence of HPVHNSCC after therapy. However, epigenetic alterations have been detected in body fluids from premalignant and early stage cancers and multiplex tests have shown promise for identification of patients with oral premalignancy. Our primary hypothesis is that multiplex testing of plasma and salivary rinses based on HPVHNSCC specific epigenetic alterations combined with high risk HPV DNA presence has potential as useful test for presence of HPVHNSCC. This project will serve as a discovery and diagnostic validation biomarker development trial for HPVHNSCC detection with potential application for assessment of disease response and development of a population based screening test.

Public Health Relevance

Human papilloma virus related head and neck cancer (HPVHNSCC) incidence is increasing rapidly in the United States, but has no available method for screening and no methods to define subclinical recurrent or persistent disease. Recent data indicate that methylation changes in DNA can be used for disease detection and that HPVHNSCC is well suited to these approaches. This project uses whole genome DNA methylation discovery techniques in combination with HPV DNA detection in salivary rinse and plasma to define a panel of biomarkers for HPVHNSCC detection.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE023347-02
Application #
8637041
Study Section
Clinical Oncology Study Section (CONC)
Program Officer
Venkatachalam, Sundaresan
Project Start
2013-04-01
Project End
2018-03-31
Budget Start
2014-04-01
Budget End
2015-03-31
Support Year
2
Fiscal Year
2014
Total Cost
$384,750
Indirect Cost
$147,250
Name
Johns Hopkins University
Department
Otolaryngology
Type
Schools of Medicine
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218