A constantly changing environment leads to challenges such as feast/famine cycles, puberty, pregnancy, and infection, which cause wide swings in the sensitivity of tissues to insulin. The healthy mammalian organism is able to compensate exquisitely, defending fasting and postprandial glucose. A major mechanism for this defense is hyperinsulinemic compensation. The latter concept reflects the appropriate increases in the fasting and postprandial blood insulin concentrations which accompany and compensate for insulin resistance. Failure of hyperinsulinemic compensation is an early sign of pre-Type 2 diabetes. Normal compensatory hyperinsulinemia can be due to either 1) increased pancreatic beta-cell function and/or 2) reduced metabolic clearance of insulin. We have obtained compelling evidence in normal animals that hyperinsulinemic compensation will occur without significant changes in arterial glucose at any hour of the day or night. The question arises, what mechanisms other than elevated glucose can contribute to the propensity of insulin to increase in the face of insulin resistance? Identification of these mechanisms will result in a deeper understanding of the pathogenesis of Type 2 diabetes. We propose to study three mechanisms for mediating hyperinsulinemic compensation.
Specific Aim 1 : We have demonstrated a powerful increase in plasma FFA levels during the night which is observed in fat-fed obese dogs. We will test the hypothesis that the 'nocturnal surge' in FFA plays a causal role in hyperinsulinemic compensation during fat-feeding induced insulin resistance. Also, we will test whether the nocturnal surge in lipolysis is related to sympathetic innervation of the visceral fat depot in the dog.
Specific Aim 2 : We will examine the hypothesis that appearance of nutrients, including glucose, in the portal circulation during postprandial absorption is a necessary component of hyperinsulinemic compensation. We will test whether the effect of nutrient absorption is mediated by specific receptors for nutrients (or hormones) in the portal circulation.
Specific Aim 3 : We will consider the 'positive feedback' hypothesis: An endogenous beta-cell secretory 'trigger' excites an early insulin response; the resulting modest hyperinsulinemia then acts on the 2-cells directly to sensitize beta-cells to further stimulation by nutrients. The latter results in more plasma insulin, which in turn further sensitizes the beta-cells, and so on - resulting in the full-blown hyperinsulinemic response to insulin resistance. Understanding the physiological underpinnings of normal compensation for insulin resistance will provide a firm basis for studying failure of compensation-part and parcel of development of Type 2 diabetes.
Significance. Type 2 diabetes mellitus is a growing problem in the U.S. and throughout the world. It is caused by the pancreas' failure to release enough of the hormone insulin when the effects of insulin are reduced to due obesity or other factors. These experiments will teach us the underlying causes of why the pancreas 'knows' to make more insulin in the obese person, and why it may lose that ability in some people, increasing the possibility of their getting diabetes. The studies will unearth new approaches to predict, prevent, treat and possibly cure Type 2 diabetes mellitus.
|Broussard, Josiane L; Nelson, Michael D; Kolka, Cathryn M et al. (2016) Rapid development of cardiac dysfunction in a canine model of insulin resistance and moderate obesity. Diabetologia 59:197-207|
|Iyer, Malini S; Bergman, Richard N; Korman, Jeremy E et al. (2016) Renal Denervation Reverses Hepatic Insulin Resistance Induced by High-Fat Diet. Diabetes :|
|Polidori, David C; Bergman, Richard N; Chung, Stephanie T et al. (2016) Hepatic and Extrahepatic Insulin Clearance Are Differentially Regulated: Results From a Novel Model-Based Analysis of Intravenous Glucose Tolerance Data. Diabetes 65:1556-64|
|Broussard, Josiane L; Castro, Ana V B; Iyer, Malini et al. (2016) Insulin access to skeletal muscle is impaired during the early stages of diet-induced obesity. Obesity (Silver Spring) 24:1922-8|
|Woolcott, Orison O; Gutierrez, Cesar; Castillo, Oscar A et al. (2016) Inverse association between altitude and obesity: A prevalence study among andean and low-altitude adult individuals of Peru. Obesity (Silver Spring) 24:929-37|
|Scarlett, Jarrad M; Rojas, Jennifer M; Matsen, Miles E et al. (2016) Central injection of fibroblast growth factor 1 induces sustained remission of diabetic hyperglycemia in rodents. Nat Med 22:800-6|
|Lee, C Christine; Watkins, Steve M; Lorenzo, Carlos et al. (2016) Branched-Chain Amino Acids and Insulin Metabolism: The Insulin Resistance Atherosclerosis Study (IRAS). Diabetes Care 39:582-8|
|Kolka, Cathryn M; Castro, Ana Valeria B; Kirkman, Erlinda L et al. (2015) Modest hyperglycemia prevents interstitial dispersion of insulin in skeletal muscle. Metabolism 64:330-7|
|Kabir, Morvarid; Iyer, Malini S; Richey, Joyce M et al. (2015) CB1R antagonist increases hepatic insulin clearance in fat-fed dogs likely via upregulation of liver adiponectin receptors. Am J Physiol Endocrinol Metab 309:E747-58|
|Dickson, J C; Liese, A D; Lorenzo, C et al. (2015) Associations of coffee consumption with markers of liver injury in the insulin resistance atherosclerosis study. BMC Gastroenterol 15:88|
Showing the most recent 10 out of 187 publications