This is a competitive renewal application in which the authors have found that growth hormone significantly facilitated the regeneration of nitric oxide synthase containing nerves after cavernous nerve injury in a group of young rats. The authors propose to examine and compare the response to cavernous nerve injury in a group of both young and old rats and to study the effect of growth hormone and its mediator insulin-like growth factor system on the erectile mechanism. If proved beneficial in the proposed experiments, clinical application of growth hormone or IGF could become an important preventative and therapeutic manure for neurogenic impotence in humans.
The specific aims are: 1: To test the hypothesis that injury to the cavernous nerve has different effects on the nonadrenergic noncholinergic vasodilator system in young and old rats. 2: To test the hypothesis that the cellular and molecular mechanisms of impotence associated with cavernous nerve injuries are due to altered gene and protein expression of NOS, TGF, NGF, IGF, FGF and adrenoreceptor in young and old rats. 3: To test the hypothesis that growth hormone facilitates regeneration of the cavernous nerve in both young and old rats. 4: To test hypothesis that the mechanism of facilitated NANC and parasympathetic nerve regeneration by growth hormone is due to production of IGF and IGFBBPs.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
2R01DK045370-03
Application #
2464717
Study Section
Special Emphasis Panel (ZRG4-UROL (01))
Project Start
1994-08-01
Project End
2001-12-31
Budget Start
1998-02-01
Budget End
1998-12-31
Support Year
3
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of California San Francisco
Department
Urology
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143
Lin, Guiting; Alwaal, Amjad; Zhang, Xiaoyu et al. (2015) Presence of stem/progenitor cells in the rat penis. Stem Cells Dev 24:264-70
Ning, Hongxiu; Lei, Hong-En; Xu, Yong-De et al. (2014) Conversion of adipose-derived stem cells into natural killer-like cells with anti-tumor activities in nude mice. PLoS One 9:e106246
Lin, Ching-Shwun; Xin, Zhongcheng; Dai, Jican et al. (2013) Stem-cell therapy for erectile dysfunction. Expert Opin Biol Ther 13:1585-97
Lin, Ching-Shwun; Lue, Tom F (2013) Defining vascular stem cells. Stem Cells Dev 22:1018-26
Lin, Ching-Shwun; Albersen, Maarten; Xin, Zhongcheng et al. (2013) Phosphodiesterase-5 expression and function in the lower urinary tract: a critical review. Urology 81:480-7
Lin, Ching-Shwun; Xin, Zhong-Cheng; Dai, Jican et al. (2013) Commonly used mesenchymal stem cell markers and tracking labels: Limitations and challenges. Histol Histopathol 28:1109-16
Ning, H; Lin, G; Lue, T F et al. (2013) A coculture system of cavernous endothelial and smooth muscle cells. Int J Impot Res 25:63-8
Lin, C-S; Xin, Z; Namiki, M et al. (2013) Direct androgen regulation of PDE5 gene or the lack thereof. Int J Impot Res 25:81-5
Ning, Hongxiu; Albersen, Maarten; Lin, Guiting et al. (2013) Effects of EdU labeling on mesenchymal stem cells. Cytotherapy 15:57-63
Wu, Alex; Lue, Tom (2012) Editorial comment. Urology 80:1373-4

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