Acute and chronic pains originating from the urinary bladder are common clinical entities affecting more than 50% of females at some time in their lives. In an attempt to understand urinary bladder hypersensitivity in a translational manner, this ongoing research project has used rodents to define basic neurophysiological elements of bladder sensation at spinal and supraspinal levels. Using urinary bladder distension (UBD)-evoked reflexes and spinal/supraspinal neuronal responses as experimental endpoints, clinically-relevant models of bladder hypersensitivity have been developed. Whereas the effect of inflammation as an exacerbator of pain has been investigated, the effects of acute stress have not. The present set of studies seeks to reverse the deficit of knowledge that exists in relation to the mechanisms of acute stress as an exacerbator of urinary bladder pain by performing systematic experimental investigations in our translational model.
Three Specific Aims are proposed:
Specific Aim #1 : To quantitatively characterize effects of acute footshock-induced stress on reflex responses to UBD in rats which experienced neonatal bladder inflammation.
Specific Aim #2 : To quantitatively characterize effects of acute footshock-induced stress on spinal neuronal responses to UBD in rats which experienced neonatal bladder inflammation.
Specific Aim #3 : To quantitatively determine the role of supraspinal mechanisms on footshock-induced alterations to responses to UBD in rats which experienced neonatal bladder inflammation. These studies will expand upon preliminary studies and will determine quantitatively the effects of the classic stressor, intermittent nonpainful footshock, on reflex (visceromotor) and spinal dorsal horn neuronal responses to UBD. Effects of descending modulatory influences will be assessed using transection of the spinal cord and/or focal supraspinal lesions in animals which experienced neonatal bladder inflammation and controls. The proposed studies examining acute stress-related effects in rodent model systems will give insight related to the effect of an acute exacerbating factor related to bladder pain. An improved understanding of sensory processing related to IC and of urinary bladder sensory pathways and their modulation by acute stress will result in an increased translation of basic science to therapeutics for bladder pain.

Public Health Relevance

The proposed studies examining acute stress-related effects in rodent model systems will give insight related to the effect of an acute exacerbating factor related to bladder pain. Neural pathways and mechanisms related to an acute stressor - footshock - will be defined. An improved understanding of sensory processing related to IC and of urinary bladder sensory pathways and their modulation by acute stress will result in an increased translation of basic science to therapeutics for bladder pain.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK051413-14
Application #
8527760
Study Section
Somatosensory and Chemosensory Systems Study Section (SCS)
Program Officer
Bavendam, Tamara G
Project Start
1997-07-25
Project End
2014-07-31
Budget Start
2013-08-01
Budget End
2014-07-31
Support Year
14
Fiscal Year
2013
Total Cost
$212,059
Indirect Cost
$67,309
Name
University of Alabama Birmingham
Department
Anesthesiology
Type
Schools of Medicine
DUNS #
063690705
City
Birmingham
State
AL
Country
United States
Zip Code
35294
Goodin, Burel R; Anderson, Austen J B; Freeman, Emily L et al. (2014) Intranasal Oxytocin Administration is Associated with Enhanced Endogenous Pain Inhibition and Reduced Negative Mood States. Clin J Pain :
Shaffer, Amber D; Ball, Chelsea L; Robbins, Meredith T et al. (2011) Effects of acute adult and early-in-life bladder inflammation on bladder neuropeptides in adult female rats. BMC Urol 11:18
DeBerry, Jennifer; Randich, Alan; Shaffer, Amber D et al. (2010) Neonatal bladder inflammation produces functional changes and alters neuropeptide content in bladders of adult female rats. J Pain 11:247-55
Ness, T J; Castroman, P J; Randich, A (2009) Acute bladder inflammation differentially affects rat spinal visceral nociceptive neurons. Neurosci Lett 467:150-4
Black, L Vandy; Ness, Timothy J; Robbins, Meredith T (2009) Effects of oxytocin and prolactin on stress-induced bladder hypersensitivity in female rats. J Pain 10:1065-72
Randich, Alan; Mebane, Hannah; Ness, Timothy J (2009) Ice water testing reveals hypersensitivity in adult rats that experienced neonatal bladder inflammation: implications for painful bladder syndrome/interstitial cystitis. J Urol 182:337-42
DeBerry, Jennifer; Ness, Timothy J; Robbins, Meredith T et al. (2007) Inflammation-induced enhancement of the visceromotor reflex to urinary bladder distention: modulation by endogenous opioids and the effects of early-in-life experience with bladder inflammation. J Pain 8:914-23
Robbins, M T; DeBerry, J; Ness, T J (2007) Chronic psychological stress enhances nociceptive processing in the urinary bladder in high-anxiety rats. Physiol Behav 91:544-50
Ness, Timothy J; Elhefni, Hanaa (2004) Reliable visceromotor responses are evoked by noxious bladder distention in mice. J Urol 171:1704-8
Ness, T J; Richter, H E; Varner, R E et al. (1998) A psychophysical study of discomfort produced by repeated filling of the urinary bladder. Pain 76:61-9