Our overall objective is to elucidate the mechanisms that control formation of adipose tissues and to determine whether and how these processes are altered by treatments for metabolic diseases. Modern life has provided unparalleled access to food supplies, contributing to a worldwide epidemic of obesity and secondary negative health consequences such as diabetes, fatty liver, cardiovascular disease, and even cancer. Thus there is an urgent need to better understand the mechanisms and molecules that control formation of adipocytes and the expansion of adipose tissue. Adipose tissue is highly dynamic, expanding and shrinking in response to various homeostatic, pharmacologic and dietary stimuli. That new adipocytes form throughout life indicates the presence of a putative adipogenic stem cell. We recently identified such an adipose stem cell by designing, engineering, and studying mice that express molecular reporters in the adipose lineage. These tools enable us to visualize adipose stem cells in vivo, as well as to follow their descendents as they divide, migrate and develop into mature adipocytes. The central focus of this proposal is to use these tools to delineate the in vivo functions and molecular characteristics of our newly discovered adult adipose stem cells. We will define the mechanistic underpinnings of the adipose stem cells and we will understand how physiological and pharmacological stimuli regulate the stem population and the growth of adipose tissue. These studies are designed to elucidate new aspects of adipose biology and metabolic control, highlighting those that are particularly relevant to new therapies for obesity and diabetes.

Public Health Relevance

The ability to regulate fat storage and metabolism are fundamental processes. However, the dual epidemics of obesity and diabetes endanger millions and are altering our health care landscape. This crisis that could be addressed by identifying genes that control formation and expansion of adipose tissue. We recently identified the adipose stem cell and found that it is important in the normal maintenance of adipose tissue mass and the response to diabetes treatments. Our goal is to unravel the developmental, physiological and molecular mechanisms underlying these effects, which we believe will enhance our understanding of adipocyte biology and may lead to novel therapeutic targets for obesity and diabetes.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Research Project (R01)
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Cellular Aspects of Diabetes and Obesity Study Section (CADO)
Program Officer
Haft, Carol R
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University of Texas Sw Medical Center Dallas
Anatomy/Cell Biology
Schools of Medicine
United States
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Jiang, Yuwei; Berry, Daniel C; Jo, Ayoung et al. (2017) A PPAR? transcriptional cascade directs adipose progenitor cell-niche interaction and niche expansion. Nat Commun 8:15926
Lapid, Kfir; Graff, Jonathan M (2017) Form(ul)ation of adipocytes by lipids. Adipocyte 6:176-186
Berry, Daniel C; Jiang, Yuwei; Arpke, Robert W et al. (2017) Cellular Aging Contributes to Failure of Cold-Induced Beige Adipocyte Formation in Old Mice and Humans. Cell Metab 25:166-181
Zeve, Daniel; Millay, Douglas P; Seo, Jin et al. (2016) Exercise-Induced Skeletal Muscle Adaptations Alter the Activity of Adipose Progenitor Cells. PLoS One 11:e0152129
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Berry, Daniel C; Jiang, Yuwei; Graff, Jonathan M (2016) Mouse strains to study cold-inducible beige progenitors and beige adipocyte formation and function. Nat Commun 7:10184
Ugrankar, Rupali; Berglund, Eric; Akdemir, Fatih et al. (2015) Drosophila glucome screening identifies Ck1alpha as a regulator of mammalian glucose metabolism. Nat Commun 6:7102
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Jiang, Yuwei; Berry, Daniel C; Tang, Wei et al. (2014) Independent stem cell lineages regulate adipose organogenesis and adipose homeostasis. Cell Rep 9:1007-22
Stenesen, Drew; Suh, Jae Myoung; Seo, Jin et al. (2013) Adenosine nucleotide biosynthesis and AMPK regulate adult life span and mediate the longevity benefit of caloric restriction in flies. Cell Metab 17:101-12

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