Abstract

Direct visualization of tissue physiology and anatomy provides important information to the physician for the diagnosis and management of disease. High spatial resolution noninvasive techniques for imaging in vivo tissue structure and blood flow dynamics are currently not available as a diagnostic tool in clinical medicine. Such techniques could have a significant impact for biomedical research and patient treatment. The objective of the proposed research is to develop a high speed noninvasive optical technique, optical coherence tomography (OCT) and optical Doppler tomography (ODT), for imaging in vivo tissue structure and blood flow with high spatial resolution (2-10mum) in biological tissues. Preliminary results obtained in our laboratory have demonstrated the potential of this technology for a number of clinical applications where imaging tissue structure and monitoring hemodynamics are important. However, there are four limitations in our current OCT/ODT system: speed, resolution, penetration depth and speckle noise. The proposed research is directed toward the development of a high speed, high resolution, phase resolved OCT/ODT system for imaging tissue structure and microcirculation that overcomes these limitations.
The specific aims of this proposal are to: (1) design and develop a high speed high resolution phase resolved OCT/ODT system for tomographic imaging of in vivo tissue structure and blood flow dynamics in highly scattering biological tissues; (2) develop signal processing and image reconstruction software and hardware for phase resolved OCT/ODT; (3) image blood flow in vitro using reconstituted canine blood and in vivo using the chick chorioallantoic membrane (CAM) model to verify and optimize OCT/ODT system operation and spatial resolution; and (4) demonstrate in animal models and clinical studies how OCT/ODT can assist in diagnosis and treatment of skin tumors and port wine stain birthmarks where imaging tissue structure and monitoring blood flow are important.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
5R01EB000293-04
Application #
6682871
Study Section
Special Emphasis Panel (ZRG1-DMG (04))
Program Officer
Zhang, Yantian
Project Start
2000-01-18
Project End
2006-08-31
Budget Start
2003-01-01
Budget End
2006-08-31
Support Year
4
Fiscal Year
2003
Total Cost
$263,330
Indirect Cost

  Institution

Name
University of California Irvine
Department
Surgery
Type
Schools of Medicine
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92697

  Publications

Englhard, Anna S; Wiedmann, Maximilian; Ledderose, Georg J et al. (2018) In vivo imaging of the internal nasal valve during different conditions using optical coherence tomography. Laryngoscope 128:E105-E110
Lin, Jennifer L; Yau, Amy Y; Boyd, Jonathon et al. (2013) Real-time subglottic stenosis imaging using optical coherence tomography in the rabbit. JAMA Otolaryngol Head Neck Surg 139:502-9
Li, Xiang; Wei, Wei; Zhou, Qifa et al. (2012) Intravascular photoacoustic imaging at 35 and 80 MHz. J Biomed Opt 17:106005
Cito, Salvatore; Ahn, Yeh-Chan; Pallares, Jordi et al. (2012) Visualization and measurement of capillary-driven blood flow using spectral domain optical coherence tomography. Microfluid Nanofluidics 13:227-237
Wei, Wei; Li, Xiang; Zhou, Qifa et al. (2011) Integrated ultrasound and photoacoustic probe for co-registered intravascular imaging. J Biomed Opt 16:106001
Liu, Gangjun; Rubinstein, Marc; Saidi, Arya et al. (2011) Imaging vibrating vocal folds with a high speed 1050 nm swept source OCT and ODT. Opt Express 19:11880-9
Eom, Tae Joong; Ahn, Yeh-Chan; Kim, Chang-Seok et al. (2011) Calibration and characterization protocol for spectral-domain optical coherence tomography using fiber Bragg gratings. J Biomed Opt 16:030501
Lee, Sang-Won; Heidary, Andrew E; Yoon, David et al. (2011) Quantification of airway thickness changes in smoke-inhalation injury using in-vivo 3-D endoscopic frequency-domain optical coherence tomography. Biomed Opt Express 2:243-54
Liu, Gangjun; Qi, Wenjuan; Yu, Lingfeng et al. (2011) Real-time bulk-motion-correction free Doppler variance optical coherence tomography for choroidal capillary vasculature imaging. Opt Express 19:3657-66
Zhang, Jun; Jing, Joe; Wang, Pinghe et al. (2011) Polarization-maintaining buffered Fourier domain mode-locked swept source for optical coherence tomography. Opt Lett 36:4788-90

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