When synthetic or natural materials, such as those used in implants and tissue engineering, come in contact with living systems, they induce specific or general responses in cells and the extracellular matrix. Cells are known to respond to both the chemistry and the mechanical properties of the surface to which they adhere. This project will explore new thin films for regulating cell adhesion and controlling cell behavior. In this work, protein and cell adhesion to ultrathin films made by the polyelectrolyte multilayering technique will be investigated. The study involves the synthesis of new polymers, their assembly, and a coordinated evaluation of the response of cells to these synthetic materials. Functional groups will be introduced into the polymers to control hydrophilicity and crosslinking. Distinction will be made between the nonspecific chemistry presented to the extracellular matrix at the surface of the film, and the film interior, which will impact the mechanical properties. The purpose of this distinction is to separate contributions of nonspecific protein adsorption and film modulus-induced cellular responses to cell adhesion, differentiation, and proliferation. The three interleaving groups of Aims present increasing levels of feedback with biological systems. We start with a goal of producing a minimally adhesive background surface, then we """"""""dope"""""""" it with precise levels of adhesion-promoting protein. We continue with an eye towards controlled chemical and physical interactions between cells and substrates. Driven partly by hypothesis and partly by a quest for new materials at the biointerface, we are interested in how cellular organization responds to broad chemical and physical cues. Points of novelty include new materials compositions, migration of a promising technology to the biointerface, methods of crosslinking, and forming gradients in mechanical properties in the plane of the film.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
5R01EB006158-03
Application #
7564065
Study Section
Biomaterials and Biointerfaces Study Section (BMBI)
Program Officer
Lee, Albert
Project Start
2007-02-01
Project End
2010-12-31
Budget Start
2009-01-01
Budget End
2009-12-31
Support Year
3
Fiscal Year
2009
Total Cost
$254,553
Indirect Cost
Name
Florida State University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
790877419
City
Tallahassee
State
FL
Country
United States
Zip Code
32306
Martinez, Jessica S; Schlenoff, Joseph B; Keller 3rd, Thomas C S (2016) Collective epithelial cell sheet adhesion and migration on polyelectrolyte multilayers with uniform and gradients of compliance. Exp Cell Res 346:17-29
Pattabhi, Sudhakara Rao; Lehaf, Ali M; Schlenoff, Joseph B et al. (2015) Human mesenchymal stem cell osteoblast differentiation, ECM deposition, and biomineralization on PAH/PAA polyelectrolyte multilayers. J Biomed Mater Res A 103:1818-27
Rao Pattabhi, Sudhakara; Martinez, Jessica S; Keller 3rd, Thomas C S (2014) Decellularized ECM effects on human mesenchymal stem cell stemness and differentiation. Differentiation 88:131-43
Martinez, Jessica S; Lehaf, Ali M; Schlenoff, Joseph B et al. (2013) Cell durotaxis on polyelectrolyte multilayers with photogenerated gradients of modulus. Biomacromolecules 14:1311-20
Lehaf, Ali M; Hariri, Haifa H; Schlenoff, Joseph B (2012) Homogeneity, modulus, and viscoelasticity of polyelectrolyte multilayers by nanoindentation: refining the buildup mechanism. Langmuir 28:6348-55
Martinez, Jessica S; Keller 3rd, Thomas C S; Schlenoff, Joseph B (2011) Cytotoxicity of free versus multilayered polyelectrolytes. Biomacromolecules 12:4063-70
Lehaf, Ali M; Moussallem, Maroun D; Schlenoff, Joseph B (2011) Correlating the compliance and permeability of photo-cross-linked polyelectrolyte multilayers. Langmuir 27:4756-63
Reisch, Andreas; Moussallem, Maroun D; Schlenoff, Joseph B (2011) Electrochemically addressed cross-links in polyelectrolyte multilayers: cyclic duravoltammetry. Langmuir 27:9418-24
Hariri, Haifa H; Schlenoff, Joseph B (2010) Saloplastic Macroporous Polyelectrolyte Complexes: Cartilage Mimics. Macromolecules 43:8656-8663
Markarian, Marie Z; Schlenoff, Joseph B (2010) Effect of molecular crowding and ionic strength on the isothermal hybridization of oligonucleotides. J Phys Chem B 114:10620-7

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