Calcium phosphate (CaP) based ceramics are used in hard tissue engineering because of their excellent biocompatibility. There is a need for the development of biodegradable ceramic materials with controlled degradation kinetics that will act as a scaffold and support bone remodeling. Our long range goal is to elucidate strength loss mechanism in CaP based material and scaffold to develop bone graft for specific application. Fundamental information on controlled degradation behavior of CaP based materials to identify optimal material composition can help us design and tailor resorbable tissue engineered bone replacement based on application needs. The objective of this research is to test our central hypothesis, which is chemistry and microstructure in CaP based ceramics can modify strength loss in these materials. Our preliminary data indicate that a minimum amount of trace elements (dopants) can have significant effects on physical and mechanical properties of CaPs. Cell-materials interactions can also be influenced by the presence of trace elements.
The specific aims are 1) To investigate effects of nanoscale CaP with three different Ca to P ratios, 1.25:1, 1.33:1 and 1.5:1, through synthesis, processing, characterization and in vitro and in vivo bone cell-materials interactions. 2) To determine the effects of four dopants, Zinc, Magnesium, Silicon, and Strontium oxides in single and multi-element composition, along with three CaP ceramics with Ca:P = 1.25:1, 1.33:1 and 1.5:1 on in vitro and in vivo resorption. 3) To develop 3D interconnected tailored porosity CaP structures using rapid prototyping, with an average 300 microns pore size, and, 30 and 60 volume % porosity and verify the influence of porosity on their properties and study in vitro and in vivo interactions. In order to accomplish these aims, we will conduct a series of studies including synthesis of nanoscale CaPs with single and multi element dopants, characterize their chemical, physical and mechanical properties, and in vitro and in vivo strength loss behavior in rat and rabbit models. It is envisioned that results from the proposed study will lead to the development of CaPs with tailored degradation kinetics that can be used in spinal fusion, maxillo- and cranio-facial implants and small scale bone defect applications.

Public Health Relevance

Resorbable Calcium Phosphate Ceramics for Bone Graft Calcium phosphate (CaP) based ceramics are used in hard tissue engineering because of their excellent biocompatibility. The objective of this research is to test our central hypothesis, which is chemistry and microstructure in Calcium phosphate (CaP) based ceramics can modify strength loss in these materials. It is envisioned that results from the proposed study will lead to the development of CaPs with tailored degradation kinetics that can be used in spinal fusion, maxillo- and cranio-facial implants and small scale bone defect applications.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
5R01EB007351-03
Application #
7767703
Study Section
Musculoskeletal Tissue Engineering Study Section (MTE)
Program Officer
Hunziker, Rosemarie
Project Start
2008-03-01
Project End
2012-02-29
Budget Start
2010-03-01
Budget End
2011-02-28
Support Year
3
Fiscal Year
2010
Total Cost
$262,660
Indirect Cost
Name
Washington State University
Department
Engineering (All Types)
Type
Schools of Engineering
DUNS #
041485301
City
Pullman
State
WA
Country
United States
Zip Code
99164
Vahabzadeh, Sahar; Hack, Vaughn Kohsei; Bose, Susmita (2017) Lithium-doped ?-tricalcium phosphate: Effects on physical, mechanical and in vitro osteoblast cell-material interactions. J Biomed Mater Res B Appl Biomater 105:391-399
Hoover, Sean; Tarafder, Solaiman; Bandyopadhyay, Amit et al. (2017) Silver doped resorbable tricalcium phosphate scaffolds for bone graft applications. Mater Sci Eng C Mater Biol Appl 79:763-769
Bose, Susmita; Tarafder, Solaiman; Bandyopadhyay, Amit (2017) Effect of Chemistry on Osteogenesis and Angiogenesis Towards Bone Tissue Engineering Using 3D Printed Scaffolds. Ann Biomed Eng 45:261-272
Vahabzadeh, Sahar; Roy, Mangal; Bose, Susmita (2015) Effects of Silicon on Osteoclast Cell Mediated Degradation, In Vivo Osteogenesis and Vasculogenesis of Brushite Cement. J Mater Chem B 3:8973-8982
Ke, Dongxu; Dernell, William; Bandyopadhyay, Amit et al. (2015) Doped tricalcium phosphate scaffolds by thermal decomposition of naphthalene: Mechanical properties and in vivo osteogenesis in a rabbit femur model. J Biomed Mater Res B Appl Biomater 103:1549-59
Vahabzadeh, Sahar; Roy, Mangal; Bandyopadhyay, Amit et al. (2015) Phase stability and biological property evaluation of plasma sprayed hydroxyapatite coatings for orthopedic and dental applications. Acta Biomater 17:47-55
Tarafder, Solaiman; Dernell, William S; Bandyopadhyay, Amit et al. (2015) SrO- and MgO-doped microwave sintered 3D printed tricalcium phosphate scaffolds: mechanical properties and in vivo osteogenesis in a rabbit model. J Biomed Mater Res B Appl Biomater 103:679-90
Vahabzadeh, Sahar; Bandyopadhyay, Amit; Bose, Susmita et al. (2015) IGF-loaded silicon and zinc doped brushite cement: physico-mechanical characterization and in vivo osteogenesis evaluation. Integr Biol (Camb) 7:1561-73
Tarafder, Solaiman; Bose, Susmita (2014) Polycaprolactone-coated 3D printed tricalcium phosphate scaffolds for bone tissue engineering: in vitro alendronate release behavior and local delivery effect on in vivo osteogenesis. ACS Appl Mater Interfaces 6:9955-65
Fielding, Gary A; Smoot, Will; Bose, Susmita (2014) Effects of SiO2, SrO, MgO, and ZnO dopants in tricalcium phosphates on osteoblastic Runx2 expression. J Biomed Mater Res A 102:2417-26

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