In this 5-yr Bioengineering Research Grant (BRG), we will develop a class of biomaterials that promote healing while preventing bacterial colonization and subsequent infections. Our group comprises experts in biomaterials synthesis and characterization, controlled drug release, inflammation and immune response, biofilm dynamics, microbial pathogenesis, and antibiotic resistance. Collectively, our group will deliver to clinical reality new technologies that will prevent biofilm formation and/or disrupt existing medical biofilms.
Specific aims of this BRG will be the following:
Aim 1. Develop porous """"""""templated"""""""" constructs (PTCs) that promote healing while preventing bacterial biofilm formation.
Aim 2. Quantify, in vitro, the efficacy of various PTCs to promote healing while preventing bacterial colonization.
Aim 3. Quantify, in vivo, the efficacy of the PTCs optimized in Aim 2 to promote healing and prevent biofilm colonization. It is estimated that over 5 million artificial or prosthetic devices are implanted per annum in the U.S. alone. However, 70% of hospital-acquired infections are associated with implants or indwelling medical devices, with the case-to-fatality ratio between 5-50%. In this 5-yr Bioengineering Research Grant (BRG), we will develop a class of biomaterials that promote healing while preventing bacterial colonization and subsequent infections.

Public Health Relevance

It is estimated that over 5 million artificial or prosthetic devices are implanted per annum in the U.S. alone. However, 70% of hospital-acquired infections are associated with implants or indwelling medical devices, with the case-to-fatality ratio between 5-50%. In this 5-yr Bioengineering Research Grant (BRG), we will develop a class of biomaterials that promote healing while preventing bacterial colonization and subsequent infections.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
5R01EB007575-04
Application #
8271373
Study Section
Biomaterials and Biointerfaces Study Section (BMBI)
Program Officer
Hunziker, Rosemarie
Project Start
2009-05-01
Project End
2014-01-30
Budget Start
2012-05-01
Budget End
2014-01-30
Support Year
4
Fiscal Year
2012
Total Cost
$435,146
Indirect Cost
$149,285
Name
University of Washington
Department
Type
Other Domestic Higher Education
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
Ma, Hongyan; Katzenmeyer, Kristy N; Bryers, James D (2013) Non-invasive in situ monitoring and quantification of TOL plasmid segregational loss within Pseudomonas putida biofilms. Biotechnol Bioeng 110:2949-58
Ma, Hongyan; Darmawan, Erica T; Zhang, Min et al. (2013) Development of a poly(ether urethane) system for the controlled release of two novel anti-biofilm agents based on gallium or zinc and its efficacy to prevent bacterial biofilm formation. J Control Release 172:1035-44
Galperin, Anna; Oldinski, Rachael A; Florczyk, Stephen J et al. (2013) Integrated bi-layered scaffold for osteochondral tissue engineering. Adv Healthc Mater 2:872-83
Ma, Hongyan; Bryers, James D (2013) Non-invasive determination of conjugative transfer of plasmids bearing antibiotic-resistance genes in biofilm-bound bacteria: effects of substrate loading and antibiotic selection. Appl Microbiol Biotechnol 97:317-28
Galperin, Anna; Long, Thomas J; Garty, Shai et al. (2013) Synthesis and fabrication of a degradable poly(N-isopropyl acrylamide) scaffold for tissue engineering applications. J Biomed Mater Res A 101:775-86
Ruckh, Timothy T; Oldinski, Rachael A; Carroll, Derek A et al. (2012) Antimicrobial effects of nanofiber poly(caprolactone) tissue scaffolds releasing rifampicin. J Mater Sci Mater Med 23:1411-20
Bryers, James D; Giachelli, Cecilia M; Ratner, Buddy D (2012) Engineering biomaterials to integrate and heal: the biocompatibility paradigm shifts. Biotechnol Bioeng 109:1898-911
Linnes, J C; Mikhova, K; Bryers, J D (2012) Adhesion of Staphylococcus epidermidis to biomaterials is inhibited by fibronectin and albumin. J Biomed Mater Res A 100:1990-7
Garty, Shai; Shirakawa, Rika; Warsen, Adelaide et al. (2011) Sustained antibiotic release from an intraocular lens-hydrogel assembly for cataract surgery. Invest Ophthalmol Vis Sci 52:6109-16
Katzenmeyer, Kristy N; Bryers, James D (2011) Multivalent artificial opsonin for the recognition and phagocytosis of Gram-positive bacteria by human phagocytes. Biomaterials 32:4042-51

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