We have demonstrated that an adult rat with a complete, mid-thoracic spinal cord transection can regain full weight-bearing stepping over a range of speeds, loads, and even directions when the spinal cord is stimulated tonically to increase the excitability of the lumbosacral locomotor circuitry. Furthermore, we learned that load- bearing sensory information can serve as the controller of these complex motor tasks and that the performance of these tasks can be improved even further with pharmacological and motor training interventions. Within the first 2.5 years of the present BRP Grant, not within the five years as initially proposed, we have gathered sufficient data to demonstrate that the human lumbosacral spinal cord has similar capabilities. We have shown that an individual with a motor complete spinal cord injury can regain independent standing, assisted stepping, and even a significant level of voluntary control of the lower limbs in the presence of epidural stimulation after months of testing different spinal cord stimulation patterns and motor training. Just as important and as impressive is the recovery of significant levels of bladder control, blood pressure, and temperature regulation, and even sexual function in this patient (13a). These intriguing results compel us to begin additional efforts to overcome what are now recognized as the most critical factors limiting our progress toward making this intervention available in the clinic. Based on our results, it is clear that we can develop the capability to selectively activae combinations of neural networks that can enable standing and probably stepping with improved technology in humans with a functionally motor complete spinal cord injury. These functional improvements are even more likely to benefit those individuals that are functionally motor incomplete, but have severely impaired mobility. Our present accomplishments have been realized using technology that is three decades old and initially designed for a different purpose, i.e., pain management. Thus we are requesting funds to overcome the present technical, not physiological, limitations as expediently and as carefully as possible. More specifically, the rat limiting factors are the lack of technology for chronic implants for a high number of electrodes and the need for a more clear understanding of how these epidural stimulation arrays can modulate the spinal circuitries. To overcome these factors we need to 1) develop a device that will allow us to chronically implant an integrated electrode array and multiplexed stimulation device in rats with the necessary signal control capabilities, 2) evaluate hypotheses that will guide us to more clearly understand how to modulate the stimulation parameters to activate the desired spinal circuits, 3) develop a learning strategy to automatically optimize the stimulation parameters for a given patient to stand, step, or exert voluntary control, and 4) begin to explore the pathways through which voluntary control can be regained after a severe spinal cord injury.

Public Health Relevance

It now seems possible to develop a technology that will enable the recovery of postural and locomotor function in humans after a motor complete spinal cord injury. This technology includes the capability to stimulate the lower spinal cord in a manner that can enable a patient with complete paraplegia to stand and to step. This project outlines the newly recognized technical capabilities that must be developed to accomplish this goal.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
3R01EB007615-03S1A1
Application #
8297976
Study Section
Neurotechnology Study Section (NT)
Program Officer
Peng, Grace
Project Start
2007-08-01
Project End
2013-08-31
Budget Start
2012-04-01
Budget End
2012-08-31
Support Year
3
Fiscal Year
2012
Total Cost
$246,827
Indirect Cost
$45,579
Name
University of California Los Angeles
Department
Physiology
Type
Schools of Arts and Sciences
DUNS #
092530369
City
Los Angeles
State
CA
Country
United States
Zip Code
90095
Gad, Parag N; Roy, Roland R; Zhong, Hui et al. (2014) Initiation of bladder voiding with epidural stimulation in paralyzed, step trained rats. PLoS One 9:e108184
Angeli, Claudia A; Edgerton, V Reggie; Gerasimenko, Yury P et al. (2014) Altering spinal cord excitability enables voluntary movements after chronic complete paralysis in humans. Brain 137:1394-409
Sayenko, Dimitry G; Angeli, Claudia; Harkema, Susan J et al. (2014) Neuromodulation of evoked muscle potentials induced by epidural spinal-cord stimulation in paralyzed individuals. J Neurophysiol 111:1088-99
Gad, Parag; Lavrov, Igor; Shah, Prithvi et al. (2013) Neuromodulation of motor-evoked potentials during stepping in spinal rats. J Neurophysiol 110:1311-22
Gad, Parag; Choe, Jaehoon; Nandra, Mandheerej Singh et al. (2013) Development of a multi-electrode array for spinal cord epidural stimulation to facilitate stepping and standing after a complete spinal cord injury in adult rats. J Neuroeng Rehabil 10:2
Shah, Prithvi K; Garcia-Alias, Guillermo; Choe, Jaehoon et al. (2013) Use of quadrupedal step training to re-engage spinal interneuronal networks and improve locomotor function after spinal cord injury. Brain 136:3362-77
Musienko, Pavel; Courtine, Gregoire; Tibbs, Jameson E et al. (2012) Somatosensory control of balance during locomotion in decerebrated cat. J Neurophysiol 107:2072-82
Johnson, Will L; Jindrich, Devin L; Roy, Roland R et al. (2012) Quantitative metrics of spinal cord injury recovery in the rat using motion capture, electromyography and ground reaction force measurement. J Neurosci Methods 206:65-72
Edgerton, V R; Roy, R R (2012) A new age for rehabilitation. Eur J Phys Rehabil Med 48:99-109
Shah, Prithvi K; Song, James; Kim, Samuel et al. (2011) Rodent estrous cycle response to incomplete spinal cord injury, surgical interventions, and locomotor training. Behav Neurosci 125:996-1002

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