Small molecules such as metabolites and drugs are crucial to the biochemistry of living organisms because of their large diversity and the sheer number present. Unlike their large molecule counter parts such proteins, DNA and mRNA, most of these species are invisible in a living cell or organism because of technical difficulties. Tagging them with bulky fluorescent labels perturbs their function. Label-free optical imaging based on Raman scattering is highly desirable because it offers contrast based on the intrinsic chemical properties of molecules via molecular vibrational spectroscopy, but has been limited to low sensitivity and long acquisition times. Our group has recently developed stimulated Raman scattering (SRS) microscopy, which offers an unprecedented combination of high sensitivity, rapid image acquisition, chemical specificity and noninvasiveness. The development of SRS microscopy has generated a great of interest from the biomedical research community and studies of lipid metabolism and drug distributions in tissue are already underway in our laboratory. We propose to continue the pioneering development in order to probe lipid metabolism, including lipogenesis, lipolysis and insulin resistance. We will image not only different types of lipids, such as saturated and unsaturated lipids and cholesterol, but also key metabolites, such as glucose, ATP, ADP, and small molecule drugs as well, all in living cells and tissue without the use of perturbative labels. These new experiments will no doubt allow new observations that will enrich our knowledge of cellular biochemistry. We will also develop all-optical cancer markers and use SRS to bring about a new paradigm for intra-operative imaging of brain tumors, improving the surgical outcome by shortening surgery and allowing more precise definition of tumor margins. The widespread adoption of SRS and the discovery of new applications will revolutionize many areas of biomedical research, including lipid metabolism, pharmacokinetics and cancer diagnosis.

Public Health Relevance

The ability to visualize small molecules such as metabolites and drugs in living cells and organisms without labels will revolutionize biomedical research, particularly lipid metabolism, pharmacokinetics and cancer diagnosis. We propose to extend the recently developed stimulated Raman scattering microscopy technique to offer rapid, sensitive and noninvasive imaging based on intrinsic vibrational frequencies of molecules, in order to selectively map the distributions of lipids, protein, glucose, cholesterol, ADP, ATP, folate and small molecule drugs, among other species. This technology will make an unprecedented transformation of our ability to monitor biochemistry in real time in living systems, and lead to a potential breakthrough in intra-operative imaging for rapid and precise tumor identification.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
5R01EB010244-05
Application #
8531699
Study Section
Special Emphasis Panel (ZRG1-BCMB-A (51))
Program Officer
Conroy, Richard
Project Start
2009-09-30
Project End
2014-08-31
Budget Start
2013-09-01
Budget End
2014-08-31
Support Year
5
Fiscal Year
2013
Total Cost
$759,192
Indirect Cost
$267,262
Name
Harvard University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
082359691
City
Cambridge
State
MA
Country
United States
Zip Code
02138
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Hollon, Todd; Lewis, Spencer; Freudiger, Christian W et al. (2016) Improving the accuracy of brain tumor surgery via Raman-based technology. Neurosurg Focus 40:E9
Tian, Feng; Yang, Wenlong; Mordes, Daniel A et al. (2016) Monitoring peripheral nerve degeneration in ALS by label-free stimulated Raman scattering imaging. Nat Commun 7:13283
Ji, Minbiao; Lewis, Spencer; Camelo-Piragua, Sandra et al. (2015) Detection of human brain tumor infiltration with quantitative stimulated Raman scattering microscopy. Sci Transl Med 7:309ra163
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Lu, Fa-Ke; Basu, Srinjan; Igras, Vivien et al. (2015) Label-free DNA imaging in vivo with stimulated Raman scattering microscopy. Proc Natl Acad Sci U S A 112:11624-9
Zhao, Ziqing W; Roy, Rahul; Gebhardt, J Christof M et al. (2014) Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy. Proc Natl Acad Sci U S A 111:681-6
Fu, Dan; Yu, Yong; Folick, Andrew et al. (2014) In vivo metabolic fingerprinting of neutral lipids with hyperspectral stimulated Raman scattering microscopy. J Am Chem Soc 136:8820-8
Fu, Dan; Zhou, Jing; Zhu, Wenjing Suzanne et al. (2014) Imaging the intracellular distribution of tyrosine kinase inhibitors in living cells with quantitative hyperspectral stimulated Raman scattering. Nat Chem 6:614-22
Freudiger, Christian W; Yang, Wenlong; Holtom, Gary R et al. (2014) Stimulated Raman Scattering Microscopy with a Robust Fibre Laser Source. Nat Photonics 8:153-159

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