Cardiovascular disease affects 80 million people in the US and is the leading cause of death.Significant limitations of current treatments necessitate the development of novel strategies. Cardiovasculartissue engineering is an emerging field focused on the development of biological substitutes to restore,maintain, or improve tissue function. Bioengineered 3-dimensional (3D) cardiac patches can be utilizedclinically at the site of a myocardial infarction, promoting increased left ventricular contractile function. Thisstudy is focused on the fabrication of 3D heart muscle, using controlled stretch to support tissue developmentand testing the ability of cardiac patches to augment the function of infarcted myocardium. The proposed study is divided into three components. The first part of this study is focused on definingcell culture conditions to maximize the functional performance of 3D cardiac patches. The second part isfocused on developing protocols to deliver controlled stretch to the 3D cardiac patches, in order to furtherincrease the functional performance of cardiac patches. In the third part of this project, cardiac patchesfabricated using optimized cell culture conditions and conditioned using stretch, will be implanted on thesurface of infarcted hearts. The ability of the cardiac patches to augment the functional performance of failingmyocardium will be studied, as a function of post-injury time and patch implantation time. Successful completion of this study will result in the development of 3D cardiac patches which will befunctional closer to normal mammalian heart muscle and will also provide insight into the potential applicabilityof the cardiac patches in supporting functional recovery of failing myocardium.
Cardiovascular disorders remain a major medical challenge with an urgent need to develop novel therapeutic options. In this study, cardiac patches will be fabricated and tested as a platform to support the recovery of infracted hearts. Successful completion of this study will result in the development of platform technology and provide a novel treatment modality for heart failure patients, benefiting millions of patients around the world.
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