The research objective of this proposal is to develop and use optically controlled microbubbles as a microrobotic system that will be used for the microassembly of artificial tissues. The optical control platform developed here will be able to control at least 50 independent microrobots in parallel, which is ten times that of current microrobotic systems. The microrobots will be able to cooperate to accomplish micromanipulation and micro-assembly tasks that can be applied to the manipulation and micro-assembly of biological cells. The micro-assembled cells can be used to create cell and tissue cultures that can be used for artificial tissue growth, drug discovery, and drug testing. The proposed project will focus on the following specific aims: 1) parallel, independent actuation of at least 50 bubble microrobots using automated optical control;2) microassembly of cell-laden hydrogels using the bubble microrobots;3) Cell patterning, sorting, and sonoporation using the bubble microrobots;4) Microassembly of cells using the microrobots to form artificial tissues. The proposed cellular microassembly for tissue engineering will address an obstacle for the development of drug therapies: cells used for drug testing are not fully representative of in vivo cell behavior. The microrobots proposed here will enable the precise yet flexible manipulation and micro-assembly of cells for building artificial tissues, creating more realistic in vitro model, and streamlining the process of developing new drug therapies. The proposed project aligns well with the mission of the National Institute of Biomedical Imaging and Bioengineering (NIBIB). This project integrates engineering and life sciences to create microrobotic systems that can advance basic medical research and medical care. The microrobotic systems will benefit medical researchers by providing them with new tools, enabling previously unrealizable protocols and experiments. The creation of artificial tissues can improve drug discovery and testing, leading to higher-quality medical care.

Public Health Relevance

This project integrates engineering and life sciences with the goal of advancing basic medical research by creating a new microrobotic system that will enable new and novel experiments. The project will also advance medical care by exploring the creation of artificial tissues for drug testing or implantation.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
5R01EB016458-02
Application #
8517118
Study Section
Special Emphasis Panel (ZEB1-OSR-A (M1))
Program Officer
Hunziker, Rosemarie
Project Start
2012-08-01
Project End
2015-07-31
Budget Start
2013-08-01
Budget End
2014-07-31
Support Year
2
Fiscal Year
2013
Total Cost
$85,608
Indirect Cost
$22,983
Name
University of Hawaii
Department
Engineering (All Types)
Type
Schools of Engineering
DUNS #
965088057
City
Honolulu
State
HI
Country
United States
Zip Code
96822
Fan, Qihui; Hu, Wenqi; Ohta, Aaron T (2014) Laser-induced microbubble poration of localized single cells. Lab Chip 14:1572-8
Hu, Wenqi; Fan, Qihui; Ohta, Aaron T (2013) An opto-thermocapillary cell micromanipulator. Lab Chip 13:2285-91