Abstract

(Investigtor's Abstract). The objective of this study is: (1) to identify the integral membrane proteins of lens cells which bind the cytoskeletal proteins CP95, CP49, ankyrin and protein 4.1 using gel overlay and immunological probes; 2) to characterize the glycoprotein population of lens membranes and their distribution with respect to differentiation and aging, using lectin and immunological probes; (3) to determine the role of NCAM and NCadherin in lens differentiation using immunological probes and tissue culture and (4) to clone the genes for chick lens cytoskeletal proteins CP95 and CP49. The overall goal is to gain a fuller understanding of the role of the plasma membrane and cytoskeleton in lens cell differentiation. This knowledge should provide new insight into lens development and the changes associated with aging and cataract formation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY001417-18
Application #
3255939
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1977-09-01
Project End
1995-08-31
Budget Start
1991-09-01
Budget End
1992-08-31
Support Year
18
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
Wayne State University
Department
Type
Schools of Medicine
DUNS #
City
Detroit
State
MI
Country
United States
Zip Code
48202

  Publications

Bagchi, M; Ansari, S A; Katar, M et al. (1997) Nonchromatin nuclear proteins of mammalian lens epithelial cells. J Cell Biochem 64:644-50
Yao, R; Alcala, J; Maisel, H (1996) Developmental changes in glycoconjugate composition during chick lens morphogenesis. Exp Eye Res 62:419-31
Yao, R; Crossland, W; Maisel, H (1996) Electron microscopic detection of glycoconjugates in the chicken lens. Exp Eye Res 63:705-11
Sawada, K; Agata, J; Eguchi, G et al. (1995) The predicted structure of chick lens CP49 and a variant thereof, CP49ins, the first vertebrate cytoplasmic intermediate filament protein with a lamin-like insertion in helix 1B. Curr Eye Res 14:545-53
Bagchi, M; Van Wijnen, A; Katar, M et al. (1995) Sequence-specific DNA binding activities of nuclear matrix proteins of mammalian lens epithelial cells. J Cell Biochem 58:1-5
Katar, M; Alcala, J; Maisel, H (1993) NCAM of the mammalian lens. Curr Eye Res 12:191-6
Orii, H; Agata, K; Sawada, K et al. (1993) Evidence that the chick lens cytoskeletal protein CP 49 belongs to the family of intermediate filament proteins. Curr Eye Res 12:583-8
Watanabe, M; Kobayashi, H; Yao, R et al. (1992) Adhesion and junction molecules in embryonic and adult lens cell differentiation. Acta Ophthalmol Suppl :46-52
Lieska, N; Yang, H Y; Maisel, H (1991) Reconstitution of the filamentous backbone of lens beaded-chain filaments from a purified 49kD polypeptide. Curr Eye Res 10:1037-48
Dola, A; Katar, M; Hussain, P et al. (1990) Ankyrin of the ocular lens. Ophthalmic Res 22:295-301

Showing the most recent 10 out of 23 publications