Abstract

In a third revision, a productive, senior investigator requests five years of support to study cGMP in the turtle retina. The proposal has three specific aims: (1) to test the hypothesis that ganglion cell activity can modulate levels of cGMP in the retina:
this aim will be accomplished by stimulation of the optic nerve to test the hypothesis that NO can function as a retrograde transmitter; (2) to localize isoforms of NOS and investigate which specific retinal cell types show changes in cGMP activity after stimulation of glutamate receptors, cholinergic receptors, ischemia and other manipulations of cGMP-related enzymes:
this aim i s designed to indicate which cells and pathways in the retina are influenced by cGMP mechanisms; and (3) electron microscopic analysis of NOS neurons and those neurons which show changes in cGMP levels in response to atrial natriuretic factors:
this aim will provide information on the synaptic control of cGMP mechanisms.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY004785-14
Application #
2444269
Study Section
Special Emphasis Panel (ZRG1-VISC (01))
Project Start
1982-08-01
Project End
1999-06-30
Budget Start
1997-07-01
Budget End
1998-06-30
Support Year
14
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
Boston University
Department
Type
Schools of Arts and Sciences
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code
02118

  Publications

Blom, Jan; Giove, Tom; Deshpande, Monika et al. (2012) Characterization of nitric oxide signaling pathways in the mouse retina. J Comp Neurol 520:4204-17
Blom, Jan; Giove, Thomas J; Pong, Winnie W et al. (2012) Evidence for a functional adrenomedullin signaling pathway in the mouse retina. Mol Vis 18:1339-53
Giove, Thomas J; Sena-Esteves, Miguel; Eldred, William D (2010) Transduction of the inner mouse retina using AAVrh8 and AAVrh10 via intravitreal injection. Exp Eye Res 91:652-9
Giove, Thomas J; Deshpande, Monika M; Eldred, William D (2009) Identification of alternate transcripts of neuronal nitric oxide synthase in the mouse retina. J Neurosci Res 87:3134-42
Blom, Jan J; Blute, Todd A; Eldred, William D (2009) Functional localization of the nitric oxide/cGMP pathway in the salamander retina. Vis Neurosci 26:275-86
Giove, Thomas J; Deshpande, Monika M; Gagen, Christine S et al. (2009) Increased neuronal nitric oxide synthase activity in retinal neurons in early diabetic retinopathy. Mol Vis 15:2249-58
Pong, Winnie W; Eldred, William D (2009) Interactions of the gaseous neuromodulators nitric oxide, carbon monoxide, and hydrogen sulfide in the salamander retina. J Neurosci Res 87:2356-64
Xie, Z; Adamowicz, W O; Eldred, W D et al. (2006) Cellular and subcellular localization of PDE10A, a striatum-enriched phosphodiesterase. Neuroscience 139:597-607
Yu, Dou; Eldred, William D (2005) Nitric oxide stimulates gamma-aminobutyric acid release and inhibits glycine release in retina. J Comp Neurol 483:278-91
Eldred, William D; Blute, Todd A (2005) Imaging of nitric oxide in the retina. Vision Res 45:3469-86

Showing the most recent 10 out of 11 publications