Cataract formation is the most common cause of vision loss, accounting for 45% of blindness worldwide. Cataract operations cost the US Medicare system ~$5 billion annually. Epidemiological studies show that the pathogenesis of human cataracts involves genetic, environmental, and other disease-associated risk factors. Lens crystallins account for 90% of the total lens proteins and play a key role in lens transparency. Hereditary cataracts account for a significant proportion of childhood blindness, and 50% of these cataracts have a genetic basis. Much recent progress has been made in identifying point mutations in genes that encode ?, ? and ?-crystallins and lead to hereditary human cataracts at birth or an early age. Functional studies on these monogenic forms of cataract could provide important information about the etiology of age-related cataracts. ?-crystallin is an aggregate of two polypeptides ?A and ?B which are expressed in lens epithelial and fiber cells. To understand disease etiology in hereditary cataracts, we have generated knock-in mouse models expressing the ?- crystallin mutations ?A-R49C and ?B-R120G linked with human autosomal dominant hereditary cataracts, using embryonic stem cell-based technologies. These mice develop cataracts at an early stage and will be an important tool to understand disease process.
Specific Aim 1 will test the hypothesis that the ?A-crystallin mutant causes epithelial and fiber cell disruption soon after the mutant protein is expressed in the developing lens. The second Specific Aim tests the hypothesis that the interaction between mutant ?-crystallin, cytoskeletal proteins and adhesion molecules disrupts the normal development of the lens cells. The third Specific Aim will test the hypothesis that mutant ?A- or ?B-crystallin disrupt normal lens protein homeostasis leading to abnormal protein degradation. Biochemical, cell biological and genetic studies will be performed. The results of these studies will provide new insights into molecular basis of lens development and cataract formation, and may promote the development of strategies to delay or prevent cataracts.
Cataract formation - a clouding of the normally clear lens - is the most common cause of blindness worldwide. Cataract surgery is one of the largest expenses in the annual Medicare healthcare costs in the U.S. Cataracts are better known for affecting older people, but non-syndromic congenital cataracts occur at an early age and cause a significant visual impairment. Genetic and environmental factors have been found to play an important part in age-related cataracts. Crystallins are highly abundant proteins of the eye lens, and mutations in crystallins genes have been shown to be the cause of many inherited cataracts. Hereditary cataracts show clinically and genetically heterogeneous lens pathology, and the relationships between cataract etiology, morphology and underlying biochemical and cell biological mechanisms remain unclear. This project will address unanswered questions to understand the molecular mechanism for cataracts caused by two different mutations in alpha crystallin genes that cause human cataracts. Mechanistic studies of genetic mutations that cause early childhood cataracts are an important area of research particularly because of its implications to understand disease etiology for the more common age-related cataracts.
|Andley, Usha P; Goldman, Joshua W (2016) Autophagy and UPR in alpha-crystallin mutant knock-in mouse models of hereditary cataracts. Biochim Biophys Acta 1860:234-9|
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|Makley, Leah N; McMenimen, Kathryn A; DeVree, Brian T et al. (2015) Pharmacological chaperone for Î±-crystallin partially restores transparency in cataract models. Science 350:674-7|
|Andley, Usha P; Malone, James P; Townsend, R Reid (2014) In vivo substrates of the lens molecular chaperones Î±A-crystallin and Î±B-crystallin. PLoS One 9:e95507|
|Wignes, Jonathan A; Goldman, Joshua W; Weihl, Conrad C et al. (2013) p62 expression and autophagy in Î±B-crystallin R120G mutant knock-in mouse model of hereditary cataract. Exp Eye Res 115:263-73|
|Andley, Usha P; Malone, James P; Hamilton, Paul D et al. (2013) Comparative proteomic analysis identifies age-dependent increases in the abundance of specific proteins after deletion of the small heat shock proteins Î±A- and Î±B-crystallin. Biochemistry 52:2933-48|
|Andley, Usha P; Hamilton, Paul D; Ravi, Nathan et al. (2011) A knock-in mouse model for the R120G mutation of *B-crystallin recapitulates human hereditary myopathy and cataracts. PLoS One 6:e17671|
|Andley, Usha P; Malone, James P; Townsend, R Reid (2011) Inhibition of lens photodamage by UV-absorbing contact lenses. Invest Ophthalmol Vis Sci 52:8330-41|
|Andley, Usha P; Reilly, Matthew A (2010) In vivo lens deficiency of the R49C alphaA-crystallin mutant. Exp Eye Res 90:699-702|
|Watson, Gregory W; Andley, Usha P (2010) Activation of the unfolded protein response by a cataract-associated Î±A-crystallin mutation. Biochem Biophys Res Commun 401:192-6|
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