Abstract

The RPE plays a critical role in maintaining the viability of retinal neurons. This grant tests the hypothesis that FGFs and their receptors are segregated on the apical and basal surfaces of the RPE cells, and that loss of this polarization results in their proliferation. There are three specific aims, each with individual objectives. First, is the release of individual FGFs polarized in monolayers of human RPE in vitro. Second, are the distribution of high and low affinity FGF receptors polarized in human RPE monolayers. Third, do cell adhesion molecules regulate basic FGF gene expression.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY006473-12
Application #
2444295
Study Section
Visual Sciences C Study Section (VISC)
Project Start
1986-07-01
Project End
2000-06-30
Budget Start
1997-07-01
Budget End
1998-06-30
Support Year
12
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
University of California Davis
Department
Ophthalmology
Type
Schools of Medicine
DUNS #
094878337
City
Davis
State
CA
Country
United States
Zip Code
95618

  Publications

Smit-McBride, Zeljka; Oltjen, Sharon L; Lavail, Matthew M et al. (2007) A strong genetic determinant of hyperoxia-related retinal degeneration on mouse chromosome 6. Invest Ophthalmol Vis Sci 48:405-11
Alizadeh, Parvaneh; Smit-McBride, Zeljka; Oltjen, Sharon L et al. (2006) Regulation of cysteine cathepsin expression by oxidative stress in the retinal pigment epithelium/choroid of the mouse. Exp Eye Res 83:679-87
Ogawa, Tsukihiko; Boylan, Sharon A; Oltjen, Sharon L et al. (2005) Changes in the spatial expression of genes with aging in the mouse RPE/choroid. Mol Vis 11:380-6
Ida, Hisashi; Ishibashi, Kazuki; Reiser, Karen et al. (2004) Ultrastructural aging of the RPE-Bruch's membrane-choriocapillaris complex in the D-galactose-treated mouse. Invest Ophthalmol Vis Sci 45:2348-54
Dunn, K C; Marmorstein, A D; Bonilha, V L et al. (1998) Use of the ARPE-19 cell line as a model of RPE polarity: basolateral secretion of FGF5. Invest Ophthalmol Vis Sci 39:2744-9
Keithahn, M A; Aotaki-Keen, A E; Schneeberger, S A et al. (1997) Expression of fibroblast growth factor-5 by bovine choroidal endothelial cells in vitro. Invest Ophthalmol Vis Sci 38:2073-80
Bost, L M; Aotaki-Keen, A E; Hjelmeland, L M (1994) Cellular adhesion regulates bFGF gene expression in human retinal pigment epithelial cells. Exp Eye Res 58:545-52
Kitaoka, T; Aotaki-Keen, A E; Hjelmeland, L M (1994) Distribution of FGF-5 in the rhesus macaque retina. Invest Ophthalmol Vis Sci 35:3189-98
Bost, L M; Hjelmeland, L M (1993) Cell density regulates differential production of bFGF transcripts. Growth Factors 9:195-203
Ishigooka, H; Kitaoka, T; Boutilier, S B et al. (1993) Developmental expression of bFGF in the bovine retina. Invest Ophthalmol Vis Sci 34:2813-23